Department of Medicine, University of Miami, Miami, Florida, USA.
Infect Control Hosp Epidemiol. 2012 Sep;33(9):897-904. doi: 10.1086/667381. Epub 2012 Jul 24.
Potential transmission of organisms from the environment to patients is a concern, especially in enclosed settings, such as operating rooms, in which there are multiple and frequent contacts between patients, provider's hands, and environmental surfaces. Therefore, adequate disinfection of operating rooms is essential. We aimed to determine the change in both the thoroughness of environmental cleaning and the proportion of environmental surfaces within operating rooms from which pathogenic organisms were recovered.
Prospective environmental study using feedback with UV markers and environmental cultures.
A 1,500-bed county teaching hospital.
Environmental service personnel, hospital administration, and medical and nursing leadership.
The proportion of UV markers removed (cleaned) increased from 0.47 (284 of 600 markers; 95% confidence interval [CI], 0.42-0.53) at baseline to 0.82 (634 of 777 markers; 95% CI, 0.77-0.85) during the last month of observations ([Formula: see text]). Nevertheless, the percentage of samples from which pathogenic organisms (gram-negative bacilli, Staphylococcus aureus, and Enterococcus species) were recovered did not change throughout our study. Pathogens were identified on 16.6% of surfaces at baseline and 12.5% of surfaces during the follow-up period ([Formula: see text]). However, the percentage of surfaces from which gram-negative bacilli were recovered decreased from 10.7% at baseline to 2.3% during the follow-up period ([Formula: see text]).
Feedback using Gram staining of environmental cultures and UV markers was successful at improving the degree of cleaning in our operating rooms.
从环境到患者的潜在生物体传播是一个关注点，尤其是在手术室等封闭环境中，因为在这些环境中，患者、医护人员的手和环境表面之间存在着频繁的多次接触。因此，手术室的充分消毒是至关重要的。我们旨在确定手术室环境清洁的彻底性和从环境表面回收病原生物的比例的变化。
使用带有紫外线标记物的反馈和环境培养物进行前瞻性环境研究。
一家拥有 1500 张床位的县教学医院。
环境服务人员、医院管理层以及医疗和护理领导层。
紫外线标记物（已清洁）的清除比例从基线时的 0.47（600 个标记物中的 284 个；95%置信区间[CI]，0.42-0.53）增加到观察的最后一个月时的 0.82（777 个标记物中的 634 个；95%CI，0.77-0.85）。然而，在我们的研究过程中，从环境样本中回收的病原生物（革兰氏阴性杆菌、金黄色葡萄球菌和肠球菌属）的百分比并没有变化。在基线时，病原体在 16.6%的表面被识别，在随访期间，病原体在 12.5%的表面被识别。然而，从革兰氏阴性杆菌回收的表面百分比从基线时的 10.7%下降到随访期间的 2.3%。
使用环境培养物的革兰氏染色和紫外线标记物的反馈成功地提高了我们手术室的清洁程度。
