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本文引用的文献

1
Estrogen metabolism and exposure in a genotypic-phenotypic model for breast cancer risk prediction.雌激素代谢与表型在乳腺癌风险预测的基因表型模型中的暴露。
Cancer Epidemiol Biomarkers Prev. 2011 Jul;20(7):1502-15. doi: 10.1158/1055-9965.EPI-11-0060. Epub 2011 May 24.
2
Single chain fragment variable recombinant antibody functionalized gold nanoparticles for a highly sensitive colorimetric immunoassay.用于高灵敏度比色免疫分析的单链可变片段重组抗体功能化金纳米颗粒
Biosens Bioelectron. 2009 May 15;24(9):2853-7. doi: 10.1016/j.bios.2009.02.016. Epub 2009 Feb 27.
3
Reactive trityl derivatives: stabilised carbocation mass-tags for life sciences applications.反应性三苯甲基衍生物：用于生命科学应用的稳定碳正离子质量标签
Org Biomol Chem. 2008 Dec 21;6(24):4593-608. doi: 10.1039/b810600b. Epub 2008 Nov 10.
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Improvements of TArgeted multiplex mass spectrometry IMaging.靶向多重质谱成像的改进
Proteomics. 2008 Sep;8(18):3725-34. doi: 10.1002/pmic.200701150.
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Carcinogen-induced histone alteration in normal human mammary epithelial cells.致癌物诱导的正常人乳腺上皮细胞中的组蛋白改变。
Carcinogenesis. 2007 Oct;28(10):2184-92. doi: 10.1093/carcin/bgm100. Epub 2007 Apr 29.
6
Recombinant antibody piezoimmunosensors for the detection of cytochrome P450 1B1.用于检测细胞色素P450 1B1的重组抗体压电免疫传感器。
Anal Chem. 2007 Feb 15;79(4):1283-9. doi: 10.1021/ac061211a.
7
Multiplex target protein imaging in tissue sections by mass spectrometry--TAMSIM.组织切片中基于质谱的多重靶蛋白成像——TAMSIM
Rapid Commun Mass Spectrom. 2007;21(6):823-9. doi: 10.1002/rcm.2895.
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Engineered recombinant single-chain fragment variable antibody for immunosensors.用于免疫传感器的工程重组单链可变片段抗体。
Anal Chem. 2005 Nov 1;77(21):6834-42. doi: 10.1021/ac0507690.
9
Single-chain fragment variable antibody piezoimmunosensors.单链可变片段抗体压电免疫传感器
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Cytochrome P450 enzymes: novel options for cancer therapeutics.
Mol Cancer Ther. 2004 Mar;3(3):363-71.

靶向多重成像质谱法与单链片段可变区（scfv）重组抗体。

Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.

机构信息

Mass Spectrometry Research Center, School of Medicine, Vanderbilt University, Nashville, TN, USA.

出版信息

J Am Soc Mass Spectrom. 2012 Oct;23(10):1689-96. doi: 10.1007/s13361-012-0423-x. Epub 2012 Aug 7.

DOI:10.1007/s13361-012-0423-x
PMID:22869296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3525520/
Abstract

Recombinant scfv antibodies specific for CYP1A1 and CYP1B1 P450 enzymes were combined with targeted imaging mass spectrometry to simultaneously detect the P450 enzymes present in archived, paraffin-embedded, human breast cancer tissue sections. By using CYP1A1 and CYP1B1 specific scfv, each coupled to a unique reporter molecule (i.e., a mass tag) it was possible to simultaneously detect multiple antigens within a single tissue sample with high sensitivity and specificity using mass spectrometry. The capability of imaging multiple antigens at the same time is a significant advance that overcomes technical barriers encountered when using present day approaches to develop assays that can simultaneously detect more than a single antigen in the same tissue sample.

摘要

针对 CYP1A1 和 CYP1B1 P450 酶的重组 scfv 抗体与靶向成像质谱联用，可同时检测存档的石蜡包埋人乳腺癌组织切片中存在的 P450 酶。通过使用 CYP1A1 和 CYP1B1 特异性 scfv，每个 scfv 与独特的报告分子（即质量标记）结合，使用质谱法可以同时在单个组织样本中以高灵敏度和特异性检测多种抗原。同时成像多种抗原的能力是一项重大进展，克服了使用当今方法开发能够同时检测同一组织样本中多个抗原的测定时遇到的技术障碍。