靶向多重成像质谱法与单链片段可变区（scfv）重组抗体。
Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.
机构信息
Mass Spectrometry Research Center, School of Medicine, Vanderbilt University, Nashville, TN, USA.
出版信息
J Am Soc Mass Spectrom. 2012 Oct;23(10):1689-96. doi: 10.1007/s13361-012-0423-x. Epub 2012 Aug 7.
Abstract
Recombinant scfv antibodies specific for CYP1A1 and CYP1B1 P450 enzymes were combined with targeted imaging mass spectrometry to simultaneously detect the P450 enzymes present in archived, paraffin-embedded, human breast cancer tissue sections. By using CYP1A1 and CYP1B1 specific scfv, each coupled to a unique reporter molecule (i.e., a mass tag) it was possible to simultaneously detect multiple antigens within a single tissue sample with high sensitivity and specificity using mass spectrometry. The capability of imaging multiple antigens at the same time is a significant advance that overcomes technical barriers encountered when using present day approaches to develop assays that can simultaneously detect more than a single antigen in the same tissue sample.
摘要
针对 CYP1A1 和 CYP1B1 P450 酶的重组 scfv 抗体与靶向成像质谱联用，可同时检测存档的石蜡包埋人乳腺癌组织切片中存在的 P450 酶。通过使用 CYP1A1 和 CYP1B1 特异性 scfv，每个 scfv 与独特的报告分子（即质量标记）结合，使用质谱法可以同时在单个组织样本中以高灵敏度和特异性检测多种抗原。同时成像多种抗原的能力是一项重大进展，克服了使用当今方法开发能够同时检测同一组织样本中多个抗原的测定时遇到的技术障碍。
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