Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University Medical Center , 465 21st Avenue South, MRB III Suite 9160, Nashville, Tennessee 37232, United States.
Biochemistry. 2013 Jun 4;52(22):3818-28. doi: 10.1021/bi301519p. Epub 2013 Jan 16.
Matrix-assisted laser desorption ionization imaging mass spectrometry (IMS) is a relatively new imaging modality that allows mapping of a wide range of biomolecules within a thin tissue section. The technology uses a laser beam to directly desorb and ionize molecules from discrete locations on the tissue that are subsequently recorded in a mass spectrometer. IMS is distinguished by the ability to directly measure molecules in situ ranging from small metabolites to proteins, reporting hundreds to thousands of expression patterns from a single imaging experiment. This article reviews recent advances in IMS technology, applications, and experimental strategies that allow it to significantly aid in the discovery and understanding of molecular processes in biological and clinical samples.
基质辅助激光解吸电离成像质谱（IMS）是一种相对较新的成像方式，可在薄组织切片内绘制多种生物分子的图谱。该技术使用激光束直接从组织上的离散位置解吸和离子化分子，随后在质谱仪中记录。IMS 的特点是能够直接原位测量从小分子代谢物到蛋白质等各种分子，从单次成像实验中报告数百到数千种表达模式。本文综述了 IMS 技术的最新进展、应用和实验策略，这些进展和策略使得它能够显著促进生物和临床样本中分子过程的发现和理解。
