Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
Am J Trop Med Hyg. 2012 Oct;87(4):658-62. doi: 10.4269/ajtmh.2012.12-0168. Epub 2012 Aug 6.
We reported a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) that detects immunoglobulin G (IgG) in urine using rKRP42 antigen for the diagnosis of visceral leishmaniasis (VL). The ELISA was applied to study chronological change in antibody titers in five study areas in Rajshahi district, Bangladesh. A total of 585 subjects without a past VL history were examined at least three times in the 30-month follow-up period; of these subjects, 137 (23.4%) subjects became ELISA-positive at least one time during the study. Among the positive cases, 40 (29.2%) subjects developed clinical VL, and 31 (77.5%) of these subjects showed IgG titers of ≥ 1,000 U more than one time in the study period. Considering only the first ELISA results, 22 subjects with IgG titers of ≥ 1,000 U could be found, and 21 (95.5%) of these subjects turned out to be clinical cases. The high urinary IgG titers (≥ 1,000 U) will help predict possible clinical VL cases and thus, identify an outbreak in its earlier stage.
我们报道了一种使用 rKRP42 抗原检测尿液中免疫球蛋白 G（IgG）的高度敏感和特异的酶联免疫吸附试验（ELISA），用于诊断内脏利什曼病（VL）。该 ELISA 用于研究孟加拉国拉杰沙希区五个研究区域中抗体滴度的时间变化。在 30 个月的随访期间，共检查了 585 名无 VL 既往史的受试者，至少进行了 3 次检查；其中，137 名（23.4%）受试者在研究期间至少有一次 ELISA 阳性。在阳性病例中，40 名（29.2%）患者出现临床 VL，其中 31 名（77.5%）患者在研究期间 IgG 滴度≥1000 U 超过一次。仅考虑第一次 ELISA 结果，可发现 22 名 IgG 滴度≥1000 U 的患者，其中 21 名（95.5%）患者为临床病例。高尿 IgG 滴度（≥1000 U）有助于预测可能的临床 VL 病例，从而更早地识别疫情。
