Takagi Hidekazu, Islam Mohammad Zahidul, Itoh Makoto, Islam Anwar Ul, Saifuddin Ekram A R M, Hussain Sultana Monira, Hashiguchi Yoshihisa, Kimura Eisaku
Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
Am J Trop Med Hyg. 2007 May;76(5):902-5.
To detect IgG antibody in the serodiagnosis of visceral leishmaniasis (VL), a recombinant antigen rK39, which is part of a Leishmania chagasi kinesin-related protein, has been used successfully and showed high sensitivity and specificity. We report production of a recombinant protein rKRP42, which is part of an L. donovani kinesin-related protein and a homolog of rK39, and its application in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of VL. When rKRP42 and rK39 were compared, amino acid sequence analysis showed 89.3% identity and 98.7% homology, with rKRP42 having 39 more amino acids than rK39. The ELISA using rKRP42 showed a sensitivity of 94.6% (70 positive samples among 74 from VL patients) and a specificity of 99.3% (148 negative samples among 149 samples from Japanese controls), whereas the sensitivity of the commercial rK39 dipstick test was 93.2% (69 positive samples among 74 from patients with VL). The rKRP42 is a promising new antigen in developing immunodiagnostic methods for VL.
为了在内脏利什曼病（VL）的血清学诊断中检测IgG抗体，一种重组抗原rK39已成功应用，它是恰加斯利什曼原虫一种驱动蛋白相关蛋白的一部分，具有高敏感性和特异性。我们报道了一种重组蛋白rKRP42的制备，它是杜氏利什曼原虫驱动蛋白相关蛋白的一部分，是rK39的同源物，并将其应用于酶联免疫吸附测定（ELISA）诊断VL。当比较rKRP42和rK39时，氨基酸序列分析显示二者具有89.3%的同一性和98.7%的同源性，rKRP42比rK39多39个氨基酸。使用rKRP42的ELISA显示敏感性为94.6%（74例VL患者样本中有70例阳性），特异性为99.3%（149例日本对照样本中有148例阴性），而商用rK39试纸条检测的敏感性为93.2%（74例VL患者样本中有69例阳性）。rKRP42是开发VL免疫诊断方法中一种有前景的新抗原。
