Department of Histology and Anatomy, College of Oriental Medicine, Daegu Haany University, Gyeongsan 712-715, Korea.
Arch Pharm Res. 2012 Jun;35(6):1073-81. doi: 10.1007/s12272-012-0615-8. Epub 2012 Jun 30.
The objective of this study is to detect the effect of beta-glucan derived from Aureobasidium pullulans SM-2001, a UV induced mutant of A. pullulans on the ovalbumin (OVA) induced allergic asthma. The test articles were orally administered to OVA-inducing asthmatic mice 4 days after sensitization for 13 days at 31.25, 62.5 or 125 mg/kg levels. Three days after the OVA sensitization, ten mice were selected per group based on body weight and were sacrificed three days after the OVA aerosol challenge. The changes on the body weight, lung weight, total leukocytes in peripheral blood and total cells in bronchoalveolar lavage fluid (BALF) were observed with changes on the lung histopathology and histomorphometry. The results were compared with dexamethasone (DEXA) 3 mg/kg intraperitoneally treated mice. The results showed increases of body weight after the OVA aerosol challenge, lung weight, total leukocytes and eosinophils in peripheral blood, total cell numbers, neutrophil and eosinophils in BALF were detected in the OVA control compared to sham control (non-OVA). However, these changes from asthmatic responses were significantly or dose-dependently decreased in the beta-glucan-dosing groups compared to those of the OVA control. Therefore, it is concluded that beta-glucan has favorable effects on asthmatic response induced by OVA. It was found that beta-glucan 125 mg/kg showed similar or slightly lower efficacy compared with DEXA 3 mg/kg.
本研究旨在检测出芽短梗霉 SM-2001（出芽短梗霉经紫外线诱导的突变株）来源的β-葡聚糖对卵清蛋白（OVA）诱导的变应性哮喘的作用。在致敏后 4 天，将受试物以 31.25、62.5 或 125mg/kg 的剂量经口给予 OVA 诱导的哮喘小鼠，共给药 13 天。在 OVA 致敏后 3 天，根据体重选择每组 10 只小鼠，并在 OVA 气溶胶攻击后 3 天处死。观察体重、肺重、外周血总白细胞和支气管肺泡灌洗液（BALF）总细胞的变化，同时观察肺组织病理学和组织形态计量学的变化。结果与腹腔内给予地塞米松（DEXA）3mg/kg 的小鼠进行比较。结果显示，与假对照（非 OVA）相比，OVA 对照组在 OVA 气溶胶攻击后体重、肺重、外周血总白细胞和嗜酸性粒细胞、BALF 总细胞数、中性粒细胞和嗜酸性粒细胞均增加。然而，与 OVA 对照组相比，β-葡聚糖给药组的这些哮喘反应变化显著或呈剂量依赖性降低。因此，β-葡聚糖对 OVA 诱导的哮喘反应有良好的作用。结果发现，β-葡聚糖 125mg/kg 的疗效与 DEXA 3mg/kg 相似或略低。
