Min Byung Gu, Park Sang Mi, Choi Youn Woong, Ku Sae Kwang, Cho Il Je, Kim Young Woo, Byun Sung Hui, Park Chung A, Park Sook Jahr, Na MinKyun, Kim Sang Chan
College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea.
Korea United Pharm. Inc., Seoul 06116, Republic of Korea.
Evid Based Complement Alternat Med. 2020 Feb 28;2020:9135637. doi: 10.1155/2020/9135637. eCollection 2020.
(PS) is traditionally used to treat respiratory and gastrointestinal infections, dysmenorrhea, and hepatic disorders in South Africa. Coptis Rhizoma (CR) is used to treat gastroenteric disorders, cardiovascular diseases, and cancer in East Asia. In the present study, we intended to observe the possible beneficial antiasthma effects of PS and CR on the ovalbumin- (OVA-) induced asthma C57BL/6J mice. Asthma in mice was induced by OVA sensitization and subsequent boosting. PS + CR (300 and 1,000 mg/kg; PO) or dexamethasone (IP) was administered once a day for 16 days. The changes in the body weight and gains, lung weights and gross inspections, total and differential cell counts of leukocytes in bronchoalveolar lavage fluid (BALF), serum OVA-specific immunoglobulin E (OVA-sIgE) levels, interleukin-4 (IL-4) and IL-5 levels in BALF and lung tissue homogenate, and IL-4 and IL-5 mRNA levels in lung tissue homogenates were analyzed with lung histopathology: mean alveolar surface area (ASA), alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in the alveolar regions, respectively. Significant increases in lung weights, total and differential cell counts of leukocytes in BALF, serum OVA-sIgE levels, and IL-4 and IL-5 levels in BALF and lung tissue homogenate were observed in OVA control as compared to those of intact control. In addition, OVA control showed a significant decrease in mean ASA and increases in alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in alveolar regions. However, these allergic and inflammatory asthmatic changes were significantly inhibited by PS + CR in a dose-dependent manner. In this study, PS + CR showed dose-dependent beneficial effects on OVA-induced asthma in mice through anti-inflammatory and antiallergic activities. Therefore, it is expected that PS + CR have enough potential as a new therapeutic agent or as an ingredient of a medicinal agent for various allergic and inflammatory respiratory diseases including asthma.
在南非，南非钩吻（PS）传统上用于治疗呼吸道和胃肠道感染、痛经及肝脏疾病。在东亚，黄连（CR）用于治疗胃肠疾病、心血管疾病和癌症。在本研究中，我们旨在观察PS和CR对卵清蛋白（OVA）诱导的哮喘C57BL/6J小鼠可能的有益抗哮喘作用。通过OVA致敏和随后的激发诱导小鼠哮喘。每天一次给予PS + CR（300和1000 mg/kg；口服）或地塞米松（腹腔注射），持续16天。分析体重及增重、肺重量和大体检查、支气管肺泡灌洗液（BALF）中白细胞的总数和分类计数、血清OVA特异性免疫球蛋白E（OVA-sIgE）水平、BALF和肺组织匀浆中白细胞介素-4（IL-4）和IL-5水平，以及肺组织匀浆中IL-4和IL-5 mRNA水平，并进行肺组织病理学检查：平均肺泡表面积（ASA）、肺泡间隔厚度、肺泡区域浸润的炎症细胞、肥大细胞和嗜酸性粒细胞数量。与完整对照组相比，OVA对照组的肺重量、BALF中白细胞的总数和分类计数、血清OVA-sIgE水平以及BALF和肺组织匀浆中IL-4和IL-5水平显著增加。此外，OVA对照组的平均ASA显著降低，肺泡间隔厚度增加，肺泡区域浸润的炎症细胞、肥大细胞和嗜酸性粒细胞数量增加。然而，PS + CR以剂量依赖的方式显著抑制了这些过敏和炎症性哮喘变化。在本研究中，PS + CR通过抗炎和抗过敏活性对OVA诱导的小鼠哮喘显示出剂量依赖性有益作用。因此，预计PS + CR作为一种新的治疗剂或作为治疗包括哮喘在内的各种过敏性和炎症性呼吸道疾病的药物成分具有足够的潜力。
