Department of Radiobiology, National Atomic Energy Commission of Argentina, San Martín, Buenos Aires, Argentina.
Reprod Sci. 2013 Jan;20(1):103-11. doi: 10.1177/1933719112451148. Epub 2012 Aug 7.
To understand the regulation of Na(+)/H(+) exchanger regulatory factor (NHERF1) in polycystic ovarian syndrome, we studied the expression of NHERF1 in uterus of Wistar rats injected with (6 mg/kg) of dehydroepiandrosterone (DHEA) for 7 and 20 days. Immunohistochemistry analysis of NHERF1 showed a substantial shift in the intracellular localization of NHERF1 in endometrial glands and areas of luminal epithelium as early as 7 days of DHEA administration. The NHERF1 accumulated in the "Golgi apparatus area" virtually in all the glands in the 7-day protocol, and in the majority of the glands of 20-day protocol. In contrast, NHERF1 is expressed in the apical membrane and slightly in the cytoplasm of the control epithelium. The subcellular redistribution of NHERF1 could affect the sorting of proteins to the apical membrane and the organization of the apical compartment.
为了了解多囊卵巢综合征中钠/氢交换调节因子（NHERF1）的调节作用，我们研究了经（6mg/kg）脱氢表雄酮（DHEA）注射 7 和 20 天的 Wistar 大鼠子宫中 NHERF1 的表达情况。NHERF1 的免疫组织化学分析表明，早在 DHEA 给药 7 天，子宫内膜腺体内和腔上皮区域的 NHERF1 就发生了明显的细胞内定位改变。在 7 天方案的几乎所有腺体内，NHERF1 积累在“高尔基体区域”，而在 20 天方案的大多数腺体内也是如此。相比之下，NHERF1 表达于对照组上皮的顶膜和细胞质中。NHERF1 的亚细胞重新分布可能会影响蛋白质向顶膜的分拣和顶区室的组织。
