Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT), National Research Council of Argentina (CONICET), Mendoza, Argentina.
Histochem Cell Biol. 2010 Dec;134(6):623-30. doi: 10.1007/s00418-010-0762-2. Epub 2010 Nov 16.
In breast cancer cell lines, the Na(+)/H(+) exchanger regulator factor 1 (NHERF1) gene is regulated at the transcriptional level by estrogens, the protein expression levels correlate with the presence of estrogen receptors and the effect is blocked by anti-estrogens. However, there is limited information regarding the regulation of NHERF1 by estrogens in normal colon tissue. The NHERF1 protein has an important role in the maintenance of the intestine ultrastructure. NHERF1-deficient mice showed defects in the intestinal microvilli as well as molecular alterations in brush border membrane proteins. Here, we have studied the expression of NHERF1 in normal rat colon and uterus during the reproductive cycle of Wistar rats. We found that NHERF1 expression in rat colon during the estral cycle is modified by estrogen levels: higher expression of NHERF1 was observed during the proestrous and estrous stages and lower expression in diestrous 1 when estrogen levels decreased. In uterus, NHERF1 was expressed in the apical region of the luminal epithelium and glands in all stages of the estral cycle, and in both colon and uterus, the expression was independent of the proliferation status. Our results show that NHERF1 expression is regulated by estrogens in colon during the rat estral cycle.
在乳腺癌细胞系中，Na(+)/H(+)交换调节剂因子 1（NHERF1）基因在转录水平上受雌激素调控，蛋白表达水平与雌激素受体的存在相关，且该作用可被抗雌激素阻断。然而，关于雌激素对正常结肠组织中 NHERF1 的调控作用，信息有限。NHERF1 蛋白在维持肠超微结构中具有重要作用。NHERF1 缺陷小鼠的肠微绒毛出现缺陷，刷状缘膜蛋白也出现分子改变。在此，我们研究了 Wistar 大鼠生殖周期中正常大鼠结肠和子宫中 NHERF1 的表达。我们发现，大鼠结肠在动情周期中 NHERF1 的表达受雌激素水平的调节：在动情前期和动情期 NHERF1 的表达较高，而在动情后期 1 时雌激素水平下降，表达较低。在子宫中，NHERF1 在腔上皮和腺的顶端区域表达，在动情周期的所有阶段均有表达，且在结肠和子宫中，表达均与增殖状态无关。我们的结果表明，在大鼠动情周期中，雌激素调控结肠中 NHERF1 的表达。
