选择性检测麦胶蛋白抗体在乳糜泻和 IgA 缺乏症中的应用：另一种途径。
Selective measurement of anti-tTG antibodies in coeliac disease and IgA deficiency: an alternative pathway.
机构信息
Department of Gastroenterology, University Hospitals Coventry and Warwickshire, Clifford Bridge Road, Coventry CV2 2DX, UK.
出版信息
Postgrad Med J. 2013 Jan;89(1047):4-7. doi: 10.1136/postgradmedj-2011-130188. Epub 2012 Aug 7.
OBJECTIVE
To determine the ability of selective antibody testing to screen for coeliac disease in the presence of IgA deficiency and to define the sensitivity of a pathway using this method.
METHOD
All IgA and IgG anti-tTG tests performed at our centre between January 2008 and December 2009, using the Immunocap 250 analyser, were retrospectively reviewed. Positive results were correlated with histology. Results were used to validate our diagnostic pathway.
RESULTS
12 289 consecutive serological tests were reviewed. IgA deficient patients gave either an 'error' reading or very low response on the Immunocap 250 analyser. Subsequent testing of this sub-group demonstrated raised IgG anti-tTG antibodies in those with histologically proven coeliac disease.
CONCLUSIONS
Using our antibody screening pathway, which involves the selective use of IgG anti-tTG, sensitivity increased from 87% to 92% in those with IgA deficiency. Adoption of this pathway for coeliac screening would negate the routine screening of immunoglobulin levels, with resultant cost saving.
目的
在 IgA 缺乏的情况下，确定选择性抗体检测筛查乳糜泻的能力，并确定使用该方法的检测路径的灵敏度。
方法
回顾性分析了 2008 年 1 月至 2009 年 12 月期间在我们中心使用 Immunocap 250 分析仪进行的所有 IgA 和 IgG 抗 tTG 检测。阳性结果与组织学相关联。结果用于验证我们的诊断途径。
结果
共回顾了 12289 例连续的血清学检测。IgA 缺乏的患者在 Immunocap 250 分析仪上要么显示“错误”读数，要么反应非常低。对该亚组的进一步检测显示，在组织学证实的乳糜泻患者中，IgG 抗 tTG 抗体升高。
结论
使用我们的抗体筛查途径，选择性地使用 IgG 抗 tTG，在 IgA 缺乏的患者中，敏感性从 87%提高到 92%。采用这种乳糜泻筛查途径可以避免常规的免疫球蛋白水平筛查，从而节省成本。
Zotero 插件