[多种细胞培养抗原在间接荧光抗体试验中用于检测抗核抗体的应用]
[Use of various cell culture antigens in the indirect fluorescent antibody test for the detection of antinuclear antibodies].
作者信息
Kocabeyoğlu O, Emekdaş G, Yücel N, Ozcan N
机构信息
Ulusal Klinik Mikrobiyoloji ve Infeksiyon Hastaliklari Kongresi'nde, Mikrobiyoloji Ana Bilim Dali Oğretim Uyesi.
出版信息
Mikrobiyol Bul. 1990 Oct;24(4):321-6.
Cell culture antigens were prepared from Vero, BHK-21 and Hep-2 cells which were propagated on slide, for detection antinuclear antibody (ANA) in sera with indirect fluorescent antibody test (IFAT). ANA were investigated in 55 sera which were positive at 1/20 titer with KB IFAT ANA test kits (Virgo), by using these cell culture antigens. 50 sera (91%) with Vero antigens, 46 sera (83%) with BHK-21 antigens and 44 sera (80%) with Hep-2 antigens were found positive at 1/20 titer. As conclusion Vero cell culture which is propagated on slide can be used as antigen for detection of ANA with IFAT.
从在玻片上培养的非洲绿猴肾细胞（Vero）、幼仓鼠肾细胞（BHK - 21）和人喉癌上皮细胞（Hep - 2）制备细胞培养抗原，用于通过间接荧光抗体试验（IFAT）检测血清中的抗核抗体（ANA）。使用这些细胞培养抗原，对55份用KB IFAT ANA检测试剂盒（Virgo）在1/20滴度呈阳性的血清进行ANA检测。发现50份（91%）用Vero抗原检测的血清、46份（83%）用BHK - 21抗原检测的血清和44份（80%）用Hep - 2抗原检测的血清在1/20滴度呈阳性。结论是在玻片上培养的Vero细胞培养物可用作通过IFAT检测ANA的抗原。
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