Litovka I H, Berezovs'kyĭ V Ia, Zamors'ka T M
Fiziol Zh (1994). 2012;58(2):51-8.
We studied the influence of normobaric hyperoxia on bone metabolism in 3- and 12-month-old male Wistar rats. Maintaining young rats (3 months) during the 14-hour daily sessions under normobaric hyperoxia (90% O2) was accompanied by a significant decrease in the concentration of C-terminal propeptides of collagen type I (by 36%), the acid phosphatase activity (by 32%), an increased activity of alkaline phosphatase (by 64%), an increased concentration of pyridinoline (by 37%) and 3-CrossLaps (by 8%), glycosaminoglycans (by 72%) in the blood serum. We believe that normobaric hyperoxia (90%) is accompanied by the disturbance of collagen synthesis. In adult rats under the same conditions for 14 sessions, the concentration ofglycosaminoglicans significantly increased by 60% relative to the control values. After 28 sessions of breathing the normobaric gas mixture containing 90% O 2 this parameter increased by 195%. Breathing normobaric gas mixture containing both 40% and 90% of O2 for 14 and 28 days decreased the acid phosphatase activity and the tartrat-resistant acid phosphatase activity by 18-25%. We believe that in adult animals 90% hyperoxia does not affect the activity of osteoblasts, but breaks the link between glycosaminoglicans and collagen fibrils, decreases the activity oflysosomal enzymes which are produced by osteoclasts and which can inhibit osteogenesis.
我们研究了常压高氧对3个月和12个月大的雄性Wistar大鼠骨代谢的影响。在常压高氧（90% O₂）环境下，让幼鼠（3个月大）每天接受14小时的处理，结果发现血清中I型胶原C端前肽浓度显著降低（降低36%）、酸性磷酸酶活性降低（降低32%）、碱性磷酸酶活性升高（升高64%）、吡啶啉浓度升高（升高37%）、3-交联C端肽（升高8%）以及糖胺聚糖浓度升高（升高72%）。我们认为常压高氧（90%）会伴随着胶原合成的紊乱。在相同条件下对成年大鼠进行14次处理后，糖胺聚糖的浓度相对于对照值显著增加了60%。在呼吸含90% O₂的常压混合气体28次后，该参数增加了195%。呼吸含40%和90% O₂的常压混合气体14天和28天，酸性磷酸酶活性和抗酒石酸酸性磷酸酶活性降低了18 - 25%。我们认为在成年动物中，90%的高氧不会影响成骨细胞的活性，但会破坏糖胺聚糖与胶原纤维之间的联系，降低破骨细胞产生的可抑制骨生成的溶酶体酶的活性。
