Jin Changzhong, Zhang Fujie, Wu Lijuan, Xie Tiansheng, Cheng Yuewu, Tang Zhirong, Wu Nanping
State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Curr HIV Res. 2012 Oct;10(7):584-91. doi: 10.2174/157016212803305961.
Immune activation and IL-7/IL7 receptor (CD127) systems are significant for CD4+ T cell defect in AIDS patients. However, the association between immune activation and CD127 expression on T lymphocyte subsets in Chinese pediatric AIDS patients is not well studied. Here, we recruited a cohort of 194 Chinese pediatric AIDS patients: 135 good viral responders (GVRs) and 59 poor viral responders (PVRs). CD38high and HLA-DR+CD8+ T lymphocytes and CD127 expression on T-cell subsets were determined by flow cytometry. Viral load was detected using a quantitative RT-PCR assay. The results showed that pediatric AIDS patients had a lower memory CD4+ percentage and a higher memory CD8+ T-cell percentage. The percentage of naive CD4+T-cells was higher, especially in GVRs. The percentage of CD38high and HLA-DR+CD8+ in PVRs was higher than in GVRs and controls, being positively correlated with viral load and memory CD8+, but negatively correlated with memory CD4+ T cells. The percentage of CD127+ memory CD4+ T cells in GVRs was higher than in PVRs, but lower than in controls. CD127 expression was positively correlated with percentage of CD4+ subsets and naive CD8+ T cells, but negatively correlated with percentage of memory CD8+ T cells. Our study demonstrates that the percentages of CD38high and HLA-DR+ CD8+ T cells are good markers to evaluate immune activation of pediatric AIDS patients, and elevated immune activation has a more profound effect on memory T cells. IL- 7/CD127 system is more important to maintain the proportion of naive T cells.
免疫激活和白细胞介素-7/白细胞介素7受体（CD127）系统对于艾滋病患者的CD4+ T细胞缺陷具有重要意义。然而，中国儿童艾滋病患者免疫激活与T淋巴细胞亚群上CD127表达之间的关联尚未得到充分研究。在此，我们招募了194名中国儿童艾滋病患者队列：135名病毒反应良好者（GVR）和59名病毒反应不佳者（PVR）。通过流式细胞术测定T细胞亚群上的CD38高表达和HLA-DR+CD8+ T淋巴细胞以及CD127表达。使用定量逆转录聚合酶链反应（RT-PCR）测定法检测病毒载量。结果显示，儿童艾滋病患者的记忆性CD4+百分比更低，而记忆性CD8+ T细胞百分比更高。初始CD4+T细胞的百分比更高，尤其是在GVR中。PVR中CD38高表达和HLA-DR+CD8+的百分比高于GVR和对照组，与病毒载量和记忆性CD8+呈正相关，但与记忆性CD4+ T细胞呈负相关。GVR中CD127+记忆性CD4+ T细胞的百分比高于PVR，但低于对照组。CD127表达与CD4+亚群和初始CD8+ T细胞的百分比呈正相关，但与记忆性CD8+ T细胞的百分比呈负相关。我们的研究表明，CD38高表达和HLA-DR+ CD8+ T细胞的百分比是评估儿童艾滋病患者免疫激活的良好标志物，免疫激活升高对记忆性T细胞有更深远的影响。白细胞介素-7/CD127系统对于维持初始T细胞的比例更为重要。
