Department of Infectious Disease, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
Laboratory of Biochemistry and Biomaterials, Department of Materials, College of Chemical and Material Engineering, Quzhou University, Quzhou, Zhejiang 324000, P.R. China.
Mol Med Rep. 2020 Aug;22(2):879-885. doi: 10.3892/mmr.2020.11153. Epub 2020 May 18.
Sterile α motif and histidine/aspartic acid domain‑containing protein 1 (SAMHD1) can inhibit reverse transcription of human immunodeficiency virus‑1 (HIV‑1) by hydrolyzing intracellular deoxy‑ribonucleoside triphosphate. However, its role in HIV‑1 disease progression has not been extensively studied. To study the impacts of SAMHD1 on HIV‑1 disease progression, especially on DNA levels, we investigated SAMHD1 levels in the peripheral blood of HIV‑1 elite controllers (ECs), antiretroviral therapy (ART) naive viremic progressors (VPs) and patients with HIV‑1 receiving ART (HIV‑ARTs) compared with healthy controls. In addition, the present study analyzed the relationship between SAMHD1 and interferon‑α, immune activation and HIV‑1 DNA levels. The results of the present study demonstrated elevated SAMHD1 expression in the peripheral blood mononuclear cells of all patients withHIV‑1, but higher SAMHD1 expression in the CD4+ T cells of only ECs compared with healthy controls. Immune activation was increased in the VPs and decreased in the ECs compared with healthy controls. Substantially lower HIV‑1 DNA levels were identified in ECs compared with those in VPs and HIV‑ARTs. SAMHD1 expression was associated with low levels of immune activation. No significant correlation was observed between SAMHD1 and HIV‑1 DNA levels. Overall, the findings of the present study indicated that SAMHD1 was highly expressed in ECs, which may be associated with low immune activation levels, but was not directly related to HIV‑1 DNA levels.
sterile α motif and histidine/aspartic acid domain-containing protein 1 (SAMHD1) 可通过水解细胞内脱氧核糖核苷三磷酸来抑制人类免疫缺陷病毒-1 (HIV-1) 的逆转录。然而，其在 HIV-1 疾病进展中的作用尚未得到广泛研究。为了研究 SAMHD1 对 HIV-1 疾病进展的影响，特别是对 DNA 水平的影响，我们检测了 HIV-1 精英控制者 (ECs)、未接受抗逆转录病毒治疗 (ART) 的病毒血症进展者 (VPs) 和接受 ART 的 HIV-1 患者 (HIV-ARTs) 外周血中的 SAMHD1 水平，并与健康对照者进行了比较。此外，本研究分析了 SAMHD1 与干扰素-α、免疫激活和 HIV-1 DNA 水平之间的关系。本研究结果表明，所有 HIV-1 患者的外周血单个核细胞中 SAMHD1 表达升高，但 ECs 中 CD4+T 细胞中的 SAMHD1 表达更高，与健康对照组相比。与健康对照组相比，VPs 中的免疫激活增加，而 ECs 中的免疫激活减少。与 VPs 和 HIV-ARTs 相比，ECs 中的 HIV-1 DNA 水平显著降低。SAMHD1 表达与低水平的免疫激活相关。SAMHD1 与 HIV-1 DNA 水平之间未观察到显著相关性。总的来说，本研究结果表明，SAMHD1 在 ECs 中高表达，这可能与低水平的免疫激活有关，但与 HIV-1 DNA 水平无直接关系。
