Rajashekharaiah Vani, Koshy Abel Abraham, Koushik Amit Kumar, Kaur Harsimran, Kumari Kavita, Agrawal Madhusudan, Khatai Smrutishree, Gowda Vaishnavi, Kumar Vinay
Department of Biotechnology, Center for Post Graduate Studies, Jain University, Jayanagar, Bangalore 560 011, India.
Transfus Apher Sci. 2012 Dec;47(3):359-64. doi: 10.1016/j.transci.2012.06.014. Epub 2012 Aug 6.
The RBCs storage lesion is most carefully viewed as the sum of all the changes in RBCs occurring during the course of storage and that limit their survival.
Erythrocytes were isolated from stored blood at regular intervals. Oxidative stress markers were analyzed to determine the changes during the storage.
Antioxidant enzymes--(SOD and CAT), and SH showed insignificant variation whereas hemolysis, MDA and AOPP showed significant variations.
The oxidative stress has not successfully overridden the protection offered by the endogenous antioxidant system. Prolonged storage may result in the onset of erythrocyte deterioration. This clearly indicates that the erythrocytes are capable of attenuating ROS with 2 weeks of storage.
红细胞储存损伤可最为确切地视为储存过程中红细胞发生的所有变化之和，这些变化限制了它们的存活。
定期从储存血液中分离红细胞。分析氧化应激标志物以确定储存期间的变化。
抗氧化酶（超氧化物歧化酶和过氧化氢酶）以及巯基显示出不显著的变化，而溶血、丙二醛和晚期氧化蛋白产物显示出显著变化。
氧化应激未能成功克服内源性抗氧化系统提供的保护。长时间储存可能导致红细胞恶化。这清楚地表明红细胞在储存2周后能够减弱活性氧。
