Hsieh Carl, Rajashekharaiah Vani
Jain University, Center for Post Graduate Studies, Department of Biotechnology, Bangalore, India.
Turk J Haematol. 2017 Dec 1;34(4):328-333. doi: 10.4274/tjh.2016.0343. Epub 2016 Dec 30.
Plasma acts as a good indicator of oxidative stress in blood. L-Carnitine is an antioxidant that reduces metabolic stress in cells, thereby providing a protective effect against oxidative stress (OS). L-Carnitine as an additive in storage has not been explored. Thus, this study attempts to analyze the role of L-carnitine in blood storage solution, citrate phosphate dextrose adenine (CPDA)-1, through OS markers including antioxidant enzymes, lipid peroxidation, and protein oxidation.
Blood was collected from male Wistar rats and stored in CPDA-1 solution with L-carnitine (10 mM, 30 mM, and 60 mM: groups LC 10, LC 30, and LC 60, respectively) and without L-carnitine (control group). Plasma was isolated every 5th day and the OS markers were analyzed.
Superoxide dismutase (SOD) and sulfhydryl (SH) increased over storage in controls, LC 30, and LC 60. Catalase increased in LC 30 and LC 60 during storage. Thiobarbituric acid reactive substances (TBARS) and protein carbonyl (PrC) levels in all groups increased initially and reduced towards the end of storage. SOD and SH levels were maintained while TBARS and PrC levels increased in LC 10.
L-Carnitine was beneficial in terms of increased antioxidant capacity and SH and decreased lipid peroxidation. This forms the basis for further studies on L-carnitine as a constituent in storage solutions.
血浆是血液中氧化应激的良好指标。左旋肉碱是一种抗氧化剂，可减轻细胞内的代谢应激，从而对氧化应激（OS）起到保护作用。左旋肉碱作为储存添加剂的作用尚未得到研究。因此，本研究试图通过抗氧化酶、脂质过氧化和蛋白质氧化等氧化应激标志物，分析左旋肉碱在血液储存溶液枸橼酸盐磷酸盐葡萄糖腺嘌呤（CPDA）-1中的作用。
从雄性Wistar大鼠采集血液，分别储存在含左旋肉碱（10 mM、30 mM和60 mM：分别为LC 10组、LC 30组和LC 60组）和不含左旋肉碱（对照组）的CPDA-1溶液中。每隔5天分离血浆并分析氧化应激标志物。
在对照组、LC 30组和LC 60组中，超氧化物歧化酶（SOD）和巯基（SH）在储存过程中增加。在储存期间，LC 30组和LC 60组的过氧化氢酶增加。所有组中的硫代巴比妥酸反应性物质（TBARS）和蛋白质羰基（PrC）水平最初升高，在储存结束时降低。在LC 10组中，SOD和SH水平保持不变，而TBARS和PrC水平升高。
左旋肉碱在提高抗氧化能力和SH水平以及降低脂质过氧化方面有益。这为进一步研究左旋肉碱作为储存溶液成分奠定了基础。
