Leite J P, Bortolotto Z A, Cavalheiro E A
Laboratório de Neurologia Experimental, Escola Paulista de Medicina, São Paulo, Brazil.
Neurosci Biobehav Rev. 1990 Winter;14(4):511-7. doi: 10.1016/s0149-7634(05)80076-4.
Seizures induced by pilocarpine (PILO) have proven to be a useful procedure for investigating the basic mechanisms essential for generation, spread and motor expression of seizures in rodents. Here we report the long-term effects of PILO in rats. Following PILO (380 mg/kg, IP), 3 distinct phases were observed: 1) an acute period which lasted 1-2 days which corresponds to the pattern of repetitive seizures and status epilepticus; 2) a silent period (4-44 days) characterized by a progressive return to normal EEG and behavior; and 3) a period of recurrent seizures which started 5-45 days after PILO and lasted up to 120 days. These seizures lasted up to 50-60 sec, recurred 2-3 times per week and were more frequent during the light period of the light-dark cycle. These serial events offer a new method to induce spontaneous recurrent seizures in rats.
毛果芸香碱（PILO）诱发的癫痫发作已被证明是一种用于研究啮齿动物癫痫发作的产生、传播和运动表现所必需的基本机制的有用方法。在此，我们报告PILO对大鼠的长期影响。给予PILO（380mg/kg，腹腔注射）后，观察到3个不同阶段：1）急性期，持续1-2天，对应于重复性癫痫发作和癫痫持续状态模式；2）静止期（4-44天），其特征是脑电图和行为逐渐恢复正常；3）癫痫复发期，在给予PILO后5-45天开始，持续长达120天。这些癫痫发作持续长达50-60秒，每周复发2-3次，在明暗周期的光照期更频繁。这些系列事件提供了一种在大鼠中诱导自发性复发性癫痫发作的新方法。
