以 D-半乳糖致衰小鼠模型评价Indocalamus 叶片总黄酮的体内抗氧化活性。

In vivo antioxidant activity of total flavonoids from indocalamus leaves in aging mice caused by D-galactose.

机构信息

School of Biological and Agricultural Engineering, Jilin University, Changchun 130022, Jilin Province, PR China.

出版信息

Food Chem Toxicol. 2012 Oct;50(10):3814-8. doi: 10.1016/j.fct.2012.07.046. Epub 2012 Aug 1.

DOI:10.1016/j.fct.2012.07.046

PMID:22874925

Abstract

OBJECTIVE

The aim of this thesis is to explore antioxidant activity of total flavonoids extracted from indocalamus leaves.

METHOD

Aging mice model was established by D-galactose induction. Three groups of mice were treated with total flavonoids extracted from indocalamus leaves at doses of 20, 40 and 80 mg/kg d bw respectively. The antioxidant status in the aging mice was measured by determining the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and total anti-oxidant capability (T-AOC) in the serum and liver and malondialdehyde (MDA) content in the serum, liver and brain.

RESULTS

Compared with control group, extracts of indocalamus leaves significantly enhanced activities of SOD, GSH-Px, CAT in the serum and liver, and decreased MDA content in the serum, liver and brain at the tested doses.

CONCLUSION

Total flavonoids extracted from indocalamus leaves demonstrated the potent antioxidant activity.

摘要

目的

本论文旨在探讨从淡竹叶中提取的总黄酮的抗氧化活性。

方法

采用 D-半乳糖诱导法建立衰老小鼠模型。将三组小鼠分别用 20、40 和 80mg/kg 体重的淡竹叶总黄酮处理。通过测定血清和肝脏中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）的活性以及血清、肝脏和大脑中丙二醛（MDA）的含量来检测衰老小鼠的抗氧化状态。