从芹菜（Apium graveolens L. var. dulce）中分离得到的一种类黄酮的体外和体内抗氧化活性。
In vitro and in vivo antioxidant activities of a flavonoid isolated from celery (Apium graveolens L. var. dulce).
机构信息
State Key Laboratory of Bioreactor Engineering, Department of Food Science and Technology, East China University of Science and Technology, P. O. Box 283, East China University of Science and Technology, 130 # Meilong Rd, Shanghai 200237, P. R. China.
出版信息
Food Funct. 2014 Jan;5(1):50-6. doi: 10.1039/c3fo60273g.
In the present article, a flavonoid was separated and purified from celery leaf through ethanol extraction, column chromatography and crystallization. The product was identified as apiin by LC/ESI-MS, and its antioxidant activities were evaluated in vitro, including by 1,1-diphenl-2-picrylhyrazyl free radical (DPPH˙), superoxide radical (O2(-)˙) and hydroxyl radical (OH˙) scavenging assays. IC50 values were 68.0 μg ml(-1) in the DPPH assay, 0.39 mg ml(-1) in the O2(-)˙ assay and 48.0 μg ml(-1) in the OH˙ assay. The antioxidant activities were investigated in vivo with the use of mice models. All data were measured including the contents of maleic dialdehyde (MDA) and lipofuscin (LPF), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and the total antioxidant capacity (TAOC), in the serum, brain, heart, liver and kidney. Results showed that apiin had a remarkable scavenging activity on MDA and LPF, promoted TAOC and significantly enhanced the activities of SOD, GSH-Px and CAT.
本文采用乙醇提取、柱层析和结晶法从芹菜叶中分离纯化得到一种黄酮类化合物，通过 LC/ESI-MS 鉴定其为芹黄素。体外实验评价了芹黄素的抗氧化活性，包括清除 1,1-二苯基-2-苦基肼自由基（DPPH˙）、超氧阴离子自由基（O2(-)˙）和羟自由基（OH˙）的能力。DPPH 自由基清除实验的 IC50 值为 68.0 μg ml(-1)，O2(-)˙清除实验的 IC50 值为 0.39 mg ml(-1)，OH˙清除实验的 IC50 值为 48.0 μg ml(-1)。采用小鼠模型进行了体内抗氧化活性研究。所有数据均通过测量血清、脑、心、肝和肾中的丙二醛（MDA）和脂褐素（LPF）含量、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性以及总抗氧化能力（TAOC）来衡量。结果表明，芹黄素对 MDA 和 LPF 具有显著的清除作用，能提高 TAOC，显著增强 SOD、GSH-Px 和 CAT 的活性。
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