Minamizaki T, Yoshida H, Yumoto T
Department of Pathology, Tottori University School of Medicine, Japan.
Pathol Res Pract. 1990 Oct;186(5):633-41. doi: 10.1016/S0344-0338(11)80227-X.
Bone tumors, which consist largely of fibroblast-like cells, were categorized into ALPase-positive (3 ossifying fibromas and 2 fibroblastic osteosarcomas) and negative (4 non-ossifying fibromas and 5 MFHs) groups. They were investigated as to their ultrastructure and immunophenotype using antibodies of fibroblast markers (collagen I, III, aminopeptidase M, dipeptidylpeptidase IV and factor XIIIa), classical macrophage markers (AACT and AAT) and vimentin. In the case of the ALPase-positive group, fibroblast-like cells showed short, branching rough endoplasmic reticulum with bundles of microfibrils in their cytoplasms. They were often intermingled with osteoblastic cells particularly in proximity to osteoid tissue. Furthermore, these cells expressed fibroblast markers of collagen I, aminopeptidase M, dipeptidylpeptidase IV and factor XIIIa. Fibroblast-like cells of the ALPase-negative group more or less revealed phagosomes in addition to fibroblastic features admixed with histiocyte-like cells. They expressed classical macrophage markers, but rarely fibroblast markers. The above findings indicated that derivation from different precursor cells should be proposed between the two groups and that the tumors in the ALPase-positive group might be intimately related to a certain population of the bone marrow stromal cells.
骨肿瘤主要由成纤维细胞样细胞构成，被分为碱性磷酸酶阳性组（3例骨化性纤维瘤和2例纤维母细胞性骨肉瘤）和阴性组（4例非骨化性纤维瘤和5例恶性纤维组织细胞瘤）。使用成纤维细胞标志物（I型、III型胶原、氨肽酶M、二肽基肽酶IV和因子XIIIa）、经典巨噬细胞标志物（AACT和AAT）以及波形蛋白的抗体，对这些肿瘤的超微结构和免疫表型进行了研究。在碱性磷酸酶阳性组中，成纤维细胞样细胞显示出短而分支的粗面内质网，其细胞质中有微原纤维束。它们常与成骨细胞混合，尤其是在类骨质组织附近。此外，这些细胞表达I型胶原、氨肽酶M、二肽基肽酶IV和因子XIIIa等成纤维细胞标志物。碱性磷酸酶阴性组的成纤维细胞样细胞除了具有与组织细胞样细胞混合的成纤维细胞特征外，或多或少还显示有吞噬体。它们表达经典巨噬细胞标志物，但很少表达成纤维细胞标志物。上述发现表明，两组之间应提出源自不同前体细胞的观点，并且碱性磷酸酶阳性组中的肿瘤可能与骨髓基质细胞的特定群体密切相关。
