Yoshida H, Miyazaki S, Yumoto T
Department of Pathology, Tottori University School of Medicine, Yonago, Japan.
Acta Pathol Jpn. 1991 Aug;41(8):610-7.
Bone tumors were categorized into alkaline phosphatase (ALPase)-positive (2 ossifying fibromas, 1 benign osteoblastoma and 16 osteosarcomas) and negative (2 chondromas, 2 chondrosarcomas, 3 non-ossifying fibromas, 2 malignant fibrous histiocytomas and 6 giant cell tumors of bone) groups. Production and distribution of matrix vesicles (MVs) in the tumor tissues were examined to clarify their role in neoplastic bone formation. Four distinct types of MV were isolated primarily in ALPase positive bone tumors: empty, amorphous, crystalline and ruptured MVs. They were formed by budding off from the cytoplasmic projections of the osteoblastic tumor cells. The significance of differences in the production rate of MVs between ALPase-positive and negative bone tumors was investigated in view of the predominantly high production of MVs in ALPase-positive bone tumors. Many more mature MVs (crystalline and ruptured) were observed in the osteoblastic lesions of osteosarcoma than in the fibroblastic and MFH-like lesions, suggesting an intimate relationship with maturation and differentiation of the osteoblastic tumor cells. The above findings indicate that production of MVs is one of the diagnostic parameters for osteoblast-derived bone tumors, as well as ALPase activity, and that vesicle-induced mineralization is a major mineralization mechanism in neoplastic bone formation.
骨肿瘤被分为碱性磷酸酶（ALPase）阳性组（2例骨化性纤维瘤、1例良性成骨细胞瘤和16例骨肉瘤）和阴性组（2例软骨瘤、2例软骨肉瘤、3例非骨化性纤维瘤、2例恶性纤维组织细胞瘤和6例骨巨细胞瘤）。检测肿瘤组织中基质小泡（MVs）的产生和分布，以阐明它们在肿瘤性骨形成中的作用。主要在ALPase阳性骨肿瘤中分离出四种不同类型的MV：空泡型、无定形、结晶型和破裂型MVs。它们是由成骨细胞性肿瘤细胞的细胞质突起芽生形成的。鉴于ALPase阳性骨肿瘤中MVs的产生率主要较高，研究了ALPase阳性和阴性骨肿瘤之间MVs产生率差异的意义。在骨肉瘤的成骨细胞病变中观察到的成熟MVs（结晶型和破裂型）比在纤维母细胞和MFH样病变中更多，这表明与成骨细胞性肿瘤细胞的成熟和分化密切相关。上述发现表明，MVs的产生是成骨细胞源性骨肿瘤的诊断参数之一，与ALPase活性一样，并且小泡诱导的矿化是肿瘤性骨形成中的主要矿化机制。
