Tudball N, Beaumont A
Biochim Biophys Acta. 1979 Dec 11;588(3):285-93. doi: 10.1016/0304-4165(79)90336-2.
Following the intracerebral administration of [35S]cystathionine, the synaptosome fraction of rat brain was labelled, the greatest uptake of amino acid being associated with hypothalamus. The uptake of [35S]cystathionine by synaptosome preparations isolated from different regions of brain, was typical of that exhibited by amino acids which are not neurotransmitters. Depolarization of the synaptic membrane had no effect on the efflux of [35S]cystathionine from preloaded synaptosomes. The intracerebral administration of cystathionine resulted in an elevation of the levels of brain cyclic AMP, the effect being particularly evident in the cerebellum. Attempts to reproduce this effect in vitro were unsuccessful.
在脑内注射[35S]胱硫醚后，大鼠脑的突触体部分被标记，氨基酸的最大摄取量与下丘脑相关。从脑的不同区域分离得到的突触体制剂对[35S]胱硫醚的摄取，是那些非神经递质氨基酸所表现出的典型摄取方式。突触膜的去极化对预加载的突触体中[35S]胱硫醚的外流没有影响。脑内注射胱硫醚导致脑环磷酸腺苷水平升高，这种效应在小脑中尤为明显。在体外重现这种效应的尝试未成功。
