Baron G, Madec-Baron A, Sampérez S, Jouan P
Centre Régional de Recherche en Endocrinologie, Faculté de Médecine-Pharmacie, Rennes, France.
Prep Biochem. 1990;20(3-4):241-56. doi: 10.1080/00327489008050199.
The fetal isoenzyme of thymidine kinase was purified to apparent homogeneity from cytosols of rat fetuses liver. A two-step purification including anion exchange chromatography and affinity chromatography was developed. The purified enzyme appears as oligomeric with a relative molecular weight of 71 kDa. In denaturing media its molecular weight was 24 kDa, and its pHi 8.3.
