A rapid two-step purification of rat liver fetal thymidine kinase.
作者信息
Baron G, Madec-Baron A, Sampérez S, Jouan P
机构信息
Centre Régional de Recherche en Endocrinologie, Faculté de Médecine-Pharmacie, Rennes, France.
出版信息
Prep Biochem. 1990;20(3-4):241-56. doi: 10.1080/00327489008050199.
PMID:2287608
Abstract
The fetal isoenzyme of thymidine kinase was purified to apparent homogeneity from cytosols of rat fetuses liver. A two-step purification including anion exchange chromatography and affinity chromatography was developed. The purified enzyme appears as oligomeric with a relative molecular weight of 71 kDa. In denaturing media its molecular weight was 24 kDa, and its pHi 8.3.
摘要
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