丹酚酸抑制低氧和血清剥夺诱导的大鼠骨髓间充质干细胞凋亡。
Inhibition of hypoxia and serum deprivation-induced apoptosis by salvianolic acid in rat mesenchymal stem cells.
机构信息
Guang'an men Hospital Affiliated to China Academy of Chinese Medical Sciences, Beijing 100053, China.
出版信息
J Tradit Chin Med. 2012 Jun;32(2):222-8. doi: 10.1016/s0254-6272(13)60015-1.
OBJECTIVE
To investigate the influence and mechanism of salvianolic acid B (SalB) on apoptosis inhibition in rat bone marrow-derived mesenchymal stem cells (BMSCs) induced by hypoxia and serum deprivation (hypoxia/SD).
METHODS
SalB concentration of 0.1, 1, 10 or 100 mg/L (drug groups) were investigated for their ability to inhibit apoptosis in rat BMSCs. BMSCs in both the apoptosis model and drug groups were cultured under hypoxic conditions for 6 h, after which cell apoptosis and change in mitochondrial membrane potential (MMP) were detected using flow cytometry. Activation of caspase-3 was detected using western blot analysis.
RESULTS
Hypoxia/SD induced apoptosis in rat BMSCs. The early apoptosis rate was lower in the drug groups compared to the apoptosis model group (P < 0.05). SalB was found to inhibit the reduction in MMP and decrease the activation of caspase-3.
CONCLUSION
0.1, 1 and 10 mg/L of SalB inhibits activation of caspase-3 and early apoptosis of rat BMSCs induced by hypoxia/SD and could therefore enhance the survival rate of grafted stem cells.
目的
研究丹酚酸 B（SalB）对缺氧/血清剥夺（Hypoxia/SD）诱导的大鼠骨髓间充质干细胞（BMSCs）凋亡抑制的影响及机制。
方法
分别用 0.1、1、10 或 100 mg/L 的 SalB（药物组）浓度来研究其抑制大鼠 BMSCs 凋亡的能力。在缺氧条件下培养凋亡模型组和药物组的 BMSCs6 h，用流式细胞术检测细胞凋亡和线粒体膜电位（MMP）的变化。用 Western blot 分析检测 caspase-3 的激活。
结果
Hypoxia/SD 诱导大鼠 BMSCs 凋亡。与凋亡模型组相比，药物组的细胞早期凋亡率较低（P < 0.05）。SalB 抑制 MMP 降低和 caspase-3 的激活。
结论
0.1、1 和 10 mg/L 的 SalB 抑制了缺氧/SD 诱导的大鼠 BMSCs 中 caspase-3 的激活和早期凋亡，从而提高了移植干细胞的存活率。
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