Traditional Chinese Medicine Hospital affiliated with Xinjiang Medical University & National Clinical Research Base of Traditional Chinese Medicine, Urumqi, Xinjiang 830000, China.
J Tradit Chin Med. 2012 Jun;32(2):234-7. doi: 10.1016/s0254-6272(13)60017-5.
To examine changes in body weight and the lung inflammation factors interleukin-1beta (IL-1beta), interleukin-8 (IL-8), IL-10 and tumor necrosis factor-alpha (TNF-alpha) in a rat model of cold-dryness syndrome in Northwest (Xinjiang) China to provide a reference for treating chronic obstructive pulmonary disease (COPD) with local peculiarities.
The rat COPD model was established by intratracheal instillation of porcine pancreatic elastase (PPE) in combination with cigarette smoking (CS). The rat model of cold-dryness syndrome of COPD in the northwest of China was set up by intratracheal instillation of PPE in combination with CS and environmental cold-dryness stress. The level of IL-1beta, IL-8, IL-10 and TNF-alpha in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). The data were analyzed using the software SPSS 11.5.
(1) Body weight was less in the two model groups than that of control group (P < 0.01), PPE plus CS cold-dryness group was less than that of PPE plus CS group (P < 0.01). (2) IL-1beta in BALF significantly increased in PPE plus CS and cold-dryness group than that of control group (P < 0.01). (3) IL-8 and TNF-alpha in BALF significantly increased in PPE plus CS and cold-dryness group and PPE plus CS group than that of control group (P < 0.01).
Body weight in COPD model rats was reduced compared with controls. Cold-dryness may aggravate such a condition lung inflammation in the model was mainly manifested by an increase in IL-1beta, IL-8 and TNF-alpha levels, with no change in IL-10 levels. Cold-dryness may aggravate lung inflammation of COPD.
观察在中国西北地区（新疆）寒燥证大鼠模型中体重和肺炎症因子白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）、IL-10 和肿瘤坏死因子-α（TNF-α）的变化，为治疗具有局部特色的慢性阻塞性肺疾病（COPD）提供参考。
采用气管内滴注猪胰弹性蛋白酶（PPE）联合香烟烟雾（CS）建立大鼠 COPD 模型，采用气管内滴注 PPE 联合 CS 及环境寒燥应激建立中国西北地区 COPD 大鼠寒燥证模型。酶联免疫吸附法（ELISA）测定支气管肺泡灌洗液（BALF）中 IL-1β、IL-8、IL-10 和 TNF-α水平。采用 SPSS 11.5 软件进行数据分析。
（1）两模型组大鼠体重均低于对照组（P < 0.01），PPE 加 CS 寒燥组大鼠体重低于 PPE 加 CS 组（P < 0.01）。（2）BALF 中 IL-1β在 PPE 加 CS 组和寒燥组均显著高于对照组（P < 0.01）。（3）BALF 中 IL-8 和 TNF-α在 PPE 加 CS 组和寒燥组及 PPE 加 CS 组均显著高于对照组（P < 0.01）。
与对照组相比，COPD 模型大鼠体重减轻。寒燥可能加重模型中肺炎症，其主要表现为 IL-1β、IL-8 和 TNF-α水平升高，IL-10 水平不变。寒燥可能加重 COPD 的肺炎症。
