Gao Zhen, Li Feng-sen, Wang Jing
Traditional Chinese Medicine Hospital of Xinjiang Medical University & National Clinical Research Base of Traditional Chinese Medicine, Urumqi.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Aug;32(8):1103-6.
To observe the expressions of tumor necrosis factor a (TNF-alpha) and matrix metalloproteinase (MMP-9) in serum and matrix metalloproteinase 1 (MMP-1) mRNA in the bone tissue in chronic obstructive pulmonary disease (COPD) patients of cold dryness syndrome (CDS) in northwest China, thus providing reference for wholism treating COPD of CDS.
Twenty-one male Wistar rats were randomly divided into three groups, i.e., the normal control group, the COPD model group, and the COPD of CDS group, 7 in each group. The COPD model was established by dripping porcine pancreatic elastase (PEE) in trachea combination with cigarette smoking, and the COPD of CDS model was also set up by dripping PEE in trachea in combination with cigarette smoking and cold-dry environmental stress. Serum contents of TNF-alpha and MMP-9 were determined using ELISA. The MMP-1 mRNA expression in rats' bone tissue was detected using fluorescent quantitative PCR.
Compared with the normal control group, the serum MMP-9 level obviously increased in the COPD of CDS group (P < 0.05). The MMP-1 mRNA expression level in the bone tissue of the COPD of CDS group and the COPD model group also obviously increased (P < 0.01, P < 0.05). The MMP-1 mRNA expression level was obviously higher in the COPD of CDS group than in the COPD model group (P < 0.01). There was no statistical difference in the serum TNF-alpha level among the three groups (P > 0.05).
CDS could increase the serum MMP-9 level and the MMP-1 mRNA expression in the bone tissue, which might be one of reasons for the fact that cold and dry environment causes more bone resorption and bone degradation in COPD.
观察中国西北地区慢性阻塞性肺疾病（COPD）寒燥证患者血清中肿瘤坏死因子α（TNF-α）、基质金属蛋白酶（MMP-9）的表达及骨组织中基质金属蛋白酶1（MMP-1）mRNA的表达，为COPD寒燥证的整体治疗提供参考。
将21只雄性Wistar大鼠随机分为3组，即正常对照组、COPD模型组、COPD寒燥证组，每组7只。采用气管滴注猪胰弹性蛋白酶（PEE）联合烟熏法建立COPD模型，采用气管滴注PEE联合烟熏及寒燥环境应激建立COPD寒燥证模型。采用酶联免疫吸附测定法（ELISA）检测血清TNF-α、MMP-9含量。采用荧光定量聚合酶链反应（PCR）检测大鼠骨组织中MMP-1 mRNA表达。
与正常对照组比较，COPD寒燥证组血清MMP-9水平明显升高（P<0.05）。COPD寒燥证组和COPD模型组骨组织中MMP-1 mRNA表达水平也明显升高（P<0.01，P<0.05）。COPD寒燥证组MMP-1 mRNA表达水平明显高于COPD模型组（P<0.01）。3组血清TNF-α水平比较差异无统计学意义（P>0.05）。
寒燥证可使血清MMP-9水平及骨组织中MMP-1 mRNA表达增加，这可能是寒燥环境导致COPD患者骨吸收和骨破坏增加的原因之一。
