The Joint Graduate School of Energy and Environment, Department of Chemical Engineering, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
Water Environ Res. 2012 Jul;84(7):577-87. doi: 10.2175/106143012x13378023685637.
The effects of organic loading rate and operating temperature on the microbial diversity and performances of upflow anaerobic sludge blanket (UASB) reactors treating palm oil mill effluent (POME) were investigated. The following two UASB reactors were run in parallel for comparison: (1) under a mesophilic condition (37 degrees C) and (2) under a mesophilic condition in transition to a thermophilic condition (57 degrees C). A polymerase chain reaction (PCR)-based denaturing gradient gel electrophoresis (DGGE) analysis showed that the microbial population profiles significantly changed with the organic loading rate (OLR) and the temperature transition from the mesophilic to the thermophilic condition. Significant biomass washout was observed for the mesophilic UASB when operating at a high organic loading rate (OLR) of 9.5 g chemical oxygen demand (COD)/L.d. In contrast, the thermophilic UASB can be operated at this OLR and at a temperature of 57 degrees C with satisfactory COD removal and biogas production. The PCR-based DGGE analysis suggested that the thermophilic temperature of 57 degrees C was suitable for a number of hydrolytic, acidogenic, and acetogenic bacteria.
研究了有机负荷率和操作温度对处理棕榈油厂废水（POME）的上流式厌氧污泥床（UASB）反应器微生物多样性和性能的影响。以下两个 UASB 反应器平行运行进行比较：（1）在中温条件（37°C）下，（2）从中温条件过渡到高温条件（57°C）。基于聚合酶链反应（PCR）的变性梯度凝胶电泳（DGGE）分析表明，微生物种群谱随着有机负荷率（OLR）和从中温到高温条件的温度变化而显著变化。当在高有机负荷率（OLR）9.5 g 化学需氧量（COD）/L.d 下运行时，中温 UASB 会发生明显的生物量洗出。相比之下，高温 UASB 可以在 57°C 的温度和此 OLR 下运行，具有令人满意的 COD 去除率和沼气产量。基于 PCR 的 DGGE 分析表明，57°C 的高温温度适合许多水解、产酸和产乙酸细菌。
