Duerkop Axel, Aleksandrova Darya, Scripinets Yuliya, Yegorova Alla, Vityukova Ekateryna
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstrasse 31, 93040 Regensburg, Germany.
Ann N Y Acad Sci. 2008;1130:172-8. doi: 10.1196/annals.1430.019.
New terbium complexes of 2-oxo-4-hydroxy-quinoline-3-carboxylic acid derivatives are reported for determination of alkaline phosphatase (aPase) and codeine phosphate (CP). Complexation with terbium occurs by way of the oxygen atoms of the 4-hydroxyl group and the carbonyl group of the 3-carboxy function, respectively. The complexes are highly luminescent and do not require luminescence enhancers. The excitation maxima of the terbium chelates are at 320 nm. The assay is based on the quenching of the hypersensitive 545-nm luminescence of the terbium complexes with the ligands 4-hydroxy-1-methyl-2-oxo-1,2-dihydro-quinoline-3-carboxylic acid (5-ethyl-[1,3,4]thiadiazol-2-yl)-amide (L(1)) and 1-ethyl-4-hydroxy-2-oxo-1,2-dihydro-quinoline-3-carboxylic acid (4-trifluoromethyl-phenyl)-amide (L(2)) by phosphate ions. The assays require equimolar (1.0 microM) concentrations of Tb(3+) and of the ligand at pH 8.0 (Tris-HCl buffer). The luminescence quenching is proportional to the concentration of aPase and CP within the range of 0.1-70 mU mL(-1) and 0.3-20 microg mL(-1), respectively. The detection limits were 0.05 mU mL(-1)=40.0 pmol L(-1) of aPase and 0.120 microg mL(-1) of CP.
据报道，新型铽配合物2-氧代-4-羟基喹啉-3-羧酸衍生物可用于测定碱性磷酸酶（aPase）和磷酸可待因（CP）。铽的络合分别通过4-羟基的氧原子和3-羧基官能团的羰基进行。这些配合物具有高发光性，不需要发光增强剂。铽螯合物的激发最大值在320nm。该测定基于磷酸根离子对铽配合物与配体4-羟基-1-甲基-2-氧代-1,2-二氢喹啉-3-羧酸（5-乙基-[1,3,4]噻二唑-2-基）-酰胺（L(1)）和1-乙基-4-羟基-2-氧代-1,2-二氢喹啉-3-羧酸（4-三氟甲基苯基）-酰胺（L(2)）的545nm超灵敏发光的猝灭。该测定在pH 8.0（Tris-HCl缓冲液）下需要等摩尔（1.0 microM）浓度的Tb(3+)和配体。发光猝灭分别与aPase和CP的浓度在0.1 - 70 mU mL(-1)和0.3 - 20 microg mL(-1)范围内成正比。aPase的检测限为0.05 mU mL(-1)=40.0 pmol L(-1)，CP的检测限为0.120 microg mL(-1)。
