Wen Quan, Zhang Nan, Cao Rui-Xia, Zhou Xin-Yu, Tang Juan, Wu Neng-Biao
Key Laboratory of Ecoenvironments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science, Southwest University, Chongqing 400715, China.
Zhong Yao Cai. 2012 Mar;35(3):341-6.
To study the physiological mechanism of anti-stress of Coptic chinensis and provide theoretical basis for its cultivation and promoting its quality.
Different degrees of the range of time and intensity of UV-B radiation were set in the experiment. Used the technique of polyacrylamide gelatin vertical board electrophoresis (PAGE) to analyse the isozyme and related stress index.
The isoenzymic bands of SOD1 (Rf = 0.125), SOD2 (Rf = 0.312), CAT1 (Rf = 0.428), POD3 (Rf = 0.290), POD4 (Rf = 0.636) were induced by UV-B radiation after 3 hours, with the increase of the time of UV-B radiation, those isoenzymic bands was going to vanish or became unclear. Moreover, isoenzymic bands of CAT1 (Rf = 0.428), POD3 (Rf = 0.290) disappeared in advance under heavy intensity of UV-B radiation. Furthermore, the contents of MDA, soluble sugar, proline were higher dramatically than those of control group under UV-B radiation. However, excluding the increases of proline in UL group, the content of MDA, soluble sugar, proline of other groups commenced to decrease slowly and isoenzymic bands of soluble protein increase after 7 hours of UV-B radiation.
The increase of the expression of antioxidase isozyme, accumulation of soluble sugar, soluble protein and other antioxidase matter is induced by the short-time UV-B radiation, which can protect Coptis chinensis from being harmed by UV-B radiation. However, regulation system of Coptis chinensis are broken, metabolism is disordered, the bands of antioxidase isozyme vanish or weaken, the bands of soluble protein are increased and widened, these phenomenon is caused by 7 hours of UV-B radiation.
研究黄连抗应激的生理机制，为黄连栽培及品质提升提供理论依据。
实验设置不同时间范围和强度的UV - B辐射。采用聚丙烯酰胺凝胶垂直板电泳（PAGE）技术分析同工酶及相关应激指标。
UV - B辐射3小时后诱导出SOD1（Rf = 0.125）、SOD2（Rf = 0.312）、CAT1（Rf = 0.428）、POD3（Rf = 0.290）、POD4（Rf = 0.636）的同工酶带，随着UV - B辐射时间增加，这些同工酶带逐渐消失或变模糊。此外，在高强度UV - B辐射下，CAT1（Rf = 0.428）、POD3（Rf = 0.290）的同工酶带提前消失。而且，UV - B辐射下丙二醛（MDA）、可溶性糖、脯氨酸含量显著高于对照组。然而，除UL组脯氨酸增加外，UV - B辐射7小时后其他组MDA、可溶性糖、脯氨酸含量开始缓慢下降，可溶性蛋白质同工酶带增加。
短时间UV - B辐射诱导抗氧化酶同工酶表达增加以及可溶性糖、可溶性蛋白质等抗氧化物质积累，可保护黄连免受UV - B辐射伤害。但7小时UV - B辐射导致黄连调节系统破坏、代谢紊乱，抗氧化酶同工酶带消失或减弱，可溶性蛋白质带增加变宽。
