Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Anal Chem. 2012 Sep 4;84(17):7422-30. doi: 10.1021/ac301332k. Epub 2012 Aug 20.
An electrostatic-spray ionization (ESTASI) method has been used for mass spectrometry (MS) analysis of samples deposited in or on an insulating substrate. The ionization is induced by a capacitive coupling between an electrode and the sample. In practice, a metallic electrode is placed close to but not in direct contact with the sample. Upon application of a high voltage pulse to the electrode, an electrostatic charging of the sample occurs leading to a bipolar spray pulse. When the voltage is positive, the bipolar spray pulse consists first of cations and then of anions. This method has been applied to a wide range of geometries to emit ions from samples in a silica capillary, in a disposable pipet tip, in a polymer microchannel, or from samples deposited as droplets on a polymer plate. Fractions from capillary electrophoresis were collected on a polymer plate for ESTASI MS analysis.
静电喷雾电离 (ESTASI) 方法已被用于对沉积在绝缘衬底上或中的样品进行质谱 (MS) 分析。通过电极与样品之间的电容耦合来诱导电离。在实际操作中，将金属电极放置在靠近但不与样品直接接触的位置。当向电极施加高压脉冲时，样品会发生静电充电，从而产生双极性喷雾脉冲。当电压为正时，双极性喷雾脉冲首先由阳离子组成，然后由阴离子组成。该方法已应用于多种几何形状，从二氧化硅毛细管中的样品、一次性移液管吸头中的样品、聚合物微通道中的样品或作为液滴沉积在聚合物板上的样品中发射离子。毛细管电泳的馏分被收集在聚合物板上，用于 ESTASI MS 分析。
