大鼠光化学诱导的缺血性中风
Photochemically induced ischemic stroke in rats.
作者信息
Schmidt Antje, Hoppen Maike, Strecker Jan-Kolja, Diederich Kai, Schäbitz Wolf-Rüdiger, Schilling Matthias, Minnerup Jens
机构信息
Department of Neurology, University of Münster, Albert-Schweitzer-Campus 1, Gebäude A1, 48149, Münster, Germany.
EVK Bielefeld, Bethel, Neurologische Klinik, Burgsteig 13, 33617, Bielefeld, Germany.
出版信息
Exp Transl Stroke Med. 2012 Aug 9;4(1):13. doi: 10.1186/2040-7378-4-13.
BACKGROUND
Photothrombosis was introduced as a model of ischemic stroke by Watson et al. in 1985. In the present paper, we describe a protocol to induce photothrombotic infarcts in rats.
FINDINGS
The photosensitive dye Bengal Rose is intravenously administered and a laser beam is stereotactically positioned onto the skull. Illumination through the intact skull leads to local activation of Bengal Rose, which results in free radical formation, disturbance of endothelial function and thrombus formation in illuminated small cortical vessels.
CONCLUSIONS
Photochemically induced infarcts cause long-term sensorimotor deficits, allow long-term survival and are particularly suitable to assess the effectiveness of neuroregenerative therapies in chronic stroke studies.
背景
1985年，沃森等人将光血栓形成引入作为缺血性中风的模型。在本文中，我们描述了一种在大鼠中诱导光血栓性梗死的方案。
研究结果
静脉注射光敏染料孟加拉玫瑰红，并用激光束立体定位在颅骨上。通过完整颅骨的光照导致孟加拉玫瑰红的局部激活，这会导致自由基形成、内皮功能紊乱以及光照区域的小皮质血管中形成血栓。
结论
光化学诱导的梗死会导致长期的感觉运动功能缺陷，允许长期存活，并且特别适合在慢性中风研究中评估神经再生疗法的有效性。
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