Institute of Respiratory Disease, Department of Medical and Occupational Sciences, Univeristy of Foggia, Italy.
Rejuvenation Res. 2012 Aug;15(4):359-65. doi: 10.1089/rej.2011.1254.
Matrix metalloproteinase-9 (MMP-9) has been recognized in several types of tumor development and progression, including lung cancer, for its role in the degradation and remodeling of lung tissue. Furthermore, increased MMP-9 has been commonly described in the serum and airways of non-small cell lung cancer (NSCLC) patients.
The aim of this study was to investigate, for the first time, MMP-9 in the exhaled breath condensate (EBC) of NSCLC patients.
We enrolled 40 NSCLC patients and 40 controls affected by transudative pleural effusion.
MMP-9 concentrations were measured in the EBC, whole blood (WB), and pleural effusion (PE) of all the subjects under study using enzyme immunoassay (EIA) kits.
MMP-9 levels were found to be significantly higher in EBC, WB, and PE of NSCLC patients compared with controls. A positive correlation was observed between MMP-9 in EBC, cigarettes smoked, and stage of cancer.
Exhaled MMP-9 was elevated in NSCLC patients, especially during tumor progression, and could represent a suitable noninvasive marker in the diagnosis and monitoring of lung cancer.
基质金属蛋白酶-9（MMP-9）在几种肿瘤的发展和进展中都有作用，包括肺癌，因其在肺组织的降解和重塑中的作用。此外，非小细胞肺癌（NSCLC）患者的血清和气道中通常会增加 MMP-9。
本研究首次探讨了 NSCLC 患者呼出气冷凝液（EBC）中的 MMP-9。
我们招募了 40 名 NSCLC 患者和 40 名患有渗出性胸腔积液的对照者。
使用酶联免疫吸附测定（EIA）试剂盒测量所有研究对象的 EBC、全血（WB）和胸腔积液（PE）中的 MMP-9 浓度。
与对照组相比，NSCLC 患者的 EBC、WB 和 PE 中的 MMP-9 水平明显更高。EBC 中的 MMP-9 与吸烟量和癌症分期呈正相关。
NSCLC 患者的呼出气 MMP-9 升高，尤其是在肿瘤进展期间，可能是诊断和监测肺癌的一种合适的非侵入性标志物。
