新型筛选酿酒酵母中含硫化合物增加突变体的方法：基于颜色的 ade1 或 ade2 突变体选择。
Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of ade1 or ade2 mutants.
机构信息
Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny proezd 1, 117545 Moscow, Russia.
出版信息
J Biosci Bioeng. 2012 Dec;114(6):615-8. doi: 10.1016/j.jbiosc.2012.07.009. Epub 2012 Aug 9.
We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry.
我们使用 1-甲基-3-硝基-1-亚硝基胍诱变剂鉴定出了酿酒酵母突变体，其细胞内谷胱甘肽含量提高了 100%。该方法采用在携带 ade1 和 ade2 突变的 met30 菌株中选择颜色最深的菌落进行可视化选择。由于该方法不涉及基因工程，因此突变体适合在食品工业中使用。
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