一种筛选含硫化合物增加的酿酒酵母突变体的新方法：使用 met30 菌株的基于颜色的菌落选择。
Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of colonies using the met30 strain.
机构信息
Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny pr., 1, 117545 Moscow, Russia.
出版信息
J Biosci Bioeng. 2012 Dec;114(6):619-21. doi: 10.1016/j.jbiosc.2012.07.011. Epub 2012 Aug 11.
PMID:22889581
Abstract
Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content.
摘要
通过检测携带野生型 ADE1 和 ADE2 等位基因的酿酒酵母 met30 酵母的色素沉着强度来检测谷胱甘肽过量产生菌。在含有高蛋氨酸含量的最小生物素培养基中生长的酵母中，观察到高度着色的菌落，这些菌落是 ade2 或 ade1 突变体的表型。
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