Center of Biomolecular Chemistry, 200 Street and 21 Ave. Atabey, Playa, La Habana, Cuba.
J Pharm Biomed Anal. 2012 Nov;70:295-300. doi: 10.1016/j.jpba.2012.07.014. Epub 2012 Jul 22.
Neisseria meningitidis constitutes the main cause of meningococcal disease in infants. Serogroups A, B, C, W135, Y, and X have the higher incidence in young children and teenagers. The use of polyvalent conjugate carbohydrate-based vaccines has decreased the meningococcal infection around the world. Recently, the serogroup X has been found to be responsible of different outbreaks of meningococcal diseases, mainly in "Meningitis Belt" of Africa and the structure of the repetitive unit of the capsular polysaccharide has been confirmed through a monodimensional (13)C NMR study. No further characterization studies have been carried out, especially with the use of other nuclei. In this paper a novel method for quantification of the N. meningitidis serogroup X by proton qNMR is reported. Deep characterization of the serogroup X polysaccharide was also carried out by combination of correlation experiments involving (13)C, (1)H, and (31)P nuclei.
脑膜炎奈瑟菌是导致婴幼儿脑膜炎的主要原因。A、B、C、W135、Y 和 X 血清群在幼儿和青少年中发病率较高。使用多价结合多糖疫苗已在全球范围内降低了脑膜炎奈瑟菌的感染。最近，血清群 X 已被发现是导致非洲“脑膜炎带”不同脑膜炎球菌病爆发的原因，其荚膜多糖重复单元的结构已通过一维（13）C NMR 研究得到证实。尚未进行进一步的特征描述研究，特别是使用其他核。本文报道了一种通过质子 qNMR 定量检测脑膜炎奈瑟菌血清群 X 的新方法。还通过结合涉及（13）C、（1）H 和（31）P 核的相关实验对血清群 X 多糖进行了深入表征。
