Fernandes Mileni da Silva, Yanai Marcela Mitsuko, Martins Gisele Miyamura, Iano Flávia Godoy, Leite Aline Lima, Cestari Tânia Mary, Taga Rumio, Buzalaf Marília Afonso Rabelo, de Oliveira Rodrigo Cardoso
Department of Biological Sciences, Bauru Dental School, University of São Paulo (USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo (SP), 17012-901, Brazil.
Odontology. 2014 Jan;102(1):22-30. doi: 10.1007/s10266-012-0083-0. Epub 2012 Aug 10.
The objective of this study was to evaluate comparatively the effect of fluoride in the expression of the receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP) in alveolar bone repair in rats. We used 3 groups of male Wistar rats (n = 5/group), which received drinking water containing different doses of F (NaF): 0, 5 and 50 ppm, for 60 days before the incisors extraction. The upper incisors were extracted and the animals were killed 7, 14, 21 and 30 days after extraction. The hemi-maxillae were collected for microscopic examination (histomorphometric and immunostaining for RANKL, OPG and TRAP). Histomorphometric analysis confirmed an increase in the volume density of neoformed bone between 7 and 30 days for groups control, 5 and 50 ppm of F, with a concomitant decrease in the volume density of connective tissue and blood clot. Higher blood clot for groups 5 and 50 ppm of F at 30 days was observed. The RANKL and OPG expressions were not changed by chronic exposure to fluoride in the drinking water during the studied periods; on the other hand, TRAP expression was changed (at 7 days) by chronic exposure to fluoride (p < 0.05). It was concluded that F in high concentrations can slow the blood clot remission and bone repair, and alter the TRAP expression in the beginning of the bone tissue repair. However, a better understanding about this blood clot remission phenomenon is required.
本研究的目的是比较评估氟对大鼠牙槽骨修复中核因子κB受体活化因子配体（RANKL）、骨保护素（OPG）和抗酒石酸酸性磷酸酶（TRAP）表达的影响。我们使用了3组雄性Wistar大鼠（每组n = 5），在拔除切牙前60天，给它们饮用含不同剂量氟（氟化钠）的水：0、5和50 ppm。拔除上颌切牙，并在拔牙后7、14、21和30天处死动物。收集半侧上颌骨进行显微镜检查（对RANKL、OPG和TRAP进行组织形态计量学和免疫染色）。组织形态计量学分析证实，对照组、5 ppm氟组和50 ppm氟组在拔牙后7至30天新生骨的体积密度增加，同时结缔组织和血凝块的体积密度降低。在30天时，观察到5 ppm氟组和50 ppm氟组的血凝块更多。在研究期间，长期饮用含氟水并未改变RANKL和OPG的表达；另一方面，长期接触氟会改变TRAP的表达（在7天时）（p < 0.05）。得出的结论是，高浓度的氟会减缓血凝块的消退和骨修复，并在骨组织修复开始时改变TRAP的表达。然而，需要对这种血凝块消退现象有更深入的了解。
