Department of Medical and Health Sciences, Division of Cardiovascular Medicine, Faculty of Health Sciences, Linköping University, SE-58185 Linköping, Sweden.
Atherosclerosis. 2012 Oct;224(2):515-20. doi: 10.1016/j.atherosclerosis.2012.07.033. Epub 2012 Jul 31.
There is emerging evidence for CD8(+) T cell alterations in blood from patients with coronary artery disease (CAD). We examined whether the distribution and phenotype of CD8(+)CD56(+) T cells differed according to the clinical manifestation of CAD.
Patients with acute coronary syndrome (ACS, n = 30), stable angina (SA, n = 34) and controls (n = 36) were included. Blood was collected before and up to 12 months after referral for coronary investigation. CD8(+)CD56(+) T cells were assessed by flow cytometry for expression of surface markers, apoptosis, and intracellular expression of cytokines.
The proportions of CD8(+)CD56(+) T cells were significantly higher in both ACS and SA patients compared with controls, and remained so after 3 and 12 months. This was independent of age, sex, systemic inflammation and cytomegalovirus seropositivity. CD8(+)CD56(+) T cells differed from CD8(+)CD56(-) T cells in terms of lower CD28 expression and fewer apoptotic cells. Both CD8(+) T cell subsets were positive for interferon (IFN)-γ and tumor necrosis factor, although IFN-γ was significantly more confined to the CD8(+)CD56(+) T cells.
The persistent accumulation of CD8(+)CD56(+) T cells in ACS and SA patients share several features with immunological aging. It also contributes to a larger IFN-γ(+) pool in blood, and may thereby hypothetically drive the atherosclerotic process in a less favorable direction.
有证据表明，冠心病（CAD）患者血液中的 CD8(+)T 细胞发生了改变。我们研究了 CD8(+)CD56(+)T 细胞的分布和表型是否因 CAD 的临床表现而不同。
纳入急性冠脉综合征（ACS，n=30）、稳定型心绞痛（SA，n=34）和对照组（n=36）患者。在转诊进行冠状动脉检查之前和之后的 12 个月内采集血液。通过流式细胞术评估 CD8(+)CD56(+)T 细胞表面标志物的表达、凋亡和细胞内细胞因子的表达。
ACS 和 SA 患者的 CD8(+)CD56(+)T 细胞比例明显高于对照组，并且在 3 个月和 12 个月后仍保持如此。这与年龄、性别、全身炎症和巨细胞病毒血清阳性无关。CD8(+)CD56(+)T 细胞与 CD8(+)CD56(-)T 细胞在 CD28 表达较低和凋亡细胞较少方面有所不同。两种 CD8(+)T 细胞亚群均呈干扰素（IFN）-γ和肿瘤坏死因子阳性，尽管 IFN-γ主要局限于 CD8(+)CD56(+)T 细胞。
ACS 和 SA 患者中持续积累的 CD8(+)CD56(+)T 细胞与免疫衰老具有一些共同特征。它还促成了血液中更大的 IFN-γ（+）池，并可能因此以不太有利的方式驱动动脉粥样硬化进程。
