[采用血清杀菌试验和酶联免疫吸附测定法检测的人血清抗C群脑膜炎奈瑟菌抗体水平比较]

[Comparison on the levels of human serum antibody against Neisseria meningitidis serogroup C measured using serum bactericidal assay and ELISA].

作者信息

Zhu Bing-qing, Xu Li, Zhou Hai-jian, Shao Zhu-jun

机构信息

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2012 May;33(5):521-4.

PMID:22883183

Abstract

OBJECTIVE

To analyze the levels of human serum antibody against Neisseria meningitidis serogroup C measured by serum bactericidal assay (SBA) and ELISA.

METHODS

SBA and a modified ELISA were applied to measure the serum bactericidal titer and the specific concentration of immunoglobulin G (IgG) against meningococcal serogroup C in sera samples. Seventy-five sera were from healthy adults without undertaking vaccination while another 429 and 388 pre- and post-vaccinated sera were from 143 infants and 194 young children immunized with conjugate vaccine or polysaccharide vaccine, respectively. Correlation between serum bactericidal titer and the concentration of specific IgG against meningococcal serogroup C was analyzed.

RESULTS

The concentration of meningococcal serogroup C specific IgG in healthy adults showed a strong correlation (r=0.814 33, P<0.001) with serum bactericidal titer through linear regression analysis. Weak correlation was observed between SBA titers and IgG concentration in pre vaccinated sera of infants and children (conjugate/polysaccharide vaccine) (infants: r=0.140 64, P>0.100/r=0.2899, P<0.05; children: r=0.540 40, P<0.05/r=0.194 36, P<0.05). After immunization with 2-dose conjugate vaccine in infants and 1-dose in children, a strong correlation between the two panels of results was observed (r=0.809 38, P<0.001 and r=0.837 23, P<0.001 respectively). However after immunization with polysaccharide vaccine, the correlation between serum bactericidal titer and concentration of specific IgG was weak (r<0.50000).

CONCLUSION

Among healthy adults and post vaccinated infants or young children immunized with conjugate vaccine, the concentration of specific IgG was comparable to the serum bactericidal titer against meningococcal serogroup C. However, it was not unfavorable to use ELISA as the principal means of measuring serum antibody responses to polysaccharide vaccine for infants under 1 year old.

摘要

目的

通过血清杀菌试验（SBA）和酶联免疫吸附测定（ELISA）分析人血清中抗C群脑膜炎奈瑟菌抗体水平。

方法

采用SBA和改良的ELISA法检测血清样本中血清杀菌效价以及抗C群脑膜炎球菌免疫球蛋白G（IgG）的特异性浓度。75份血清来自未接种疫苗的健康成年人，另外429份和388份接种疫苗前后的血清分别来自143名接种结合疫苗的婴儿和194名接种多糖疫苗的幼儿。分析血清杀菌效价与抗C群脑膜炎球菌特异性IgG浓度之间的相关性。

结果

通过线性回归分析，健康成年人中C群脑膜炎球菌特异性IgG浓度与血清杀菌效价呈强相关性（r = 0.814 33，P < 0.）。在婴儿和儿童（结合疫苗/多糖疫苗）接种前的血清中，观察到SBA效价与IgG浓度之间存在弱相关性（婴儿：r = 0.140 64，P > 0.100/r = 0.2899，P < 0.05；儿童：r = 0.540 40，P < 0.05/r = 0.194 36，P < 0.05）。婴儿接种2剂结合疫苗和儿童接种1剂结合疫苗后，两组结果之间观察到强相关性（分别为r = 0.809 38，P < 0.001和r = 0.837 23，P < 0.001）。然而，接种多糖疫苗后，血清杀菌效价与特异性IgG浓度之间的相关性较弱（r < 0.50000）。