[电针内关穴和心俞穴对急性心肌缺血大鼠脊髓背根神经电活动及下丘脑室旁核去甲肾上腺素和多巴胺含量的影响]

[Effects of electroacupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia].

作者信息

Li Meng, Hu Ling, Cai Rong-lin, Wu Zi-jian, Wang Ke-ming

机构信息

Institute of Acupuncture, Moxibustion, and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China.

出版信息

Zhong Xi Yi Jie He Xue Bao. 2012 Aug;10(8):874-9. doi: 10.3736/jcim20120807.

DOI:10.3736/jcim20120807

PMID:22883403

Abstract

OBJECTIVE

To investigate the effects of electroacupuncture (EA) at Neiguan (PC6) and Xinshu (BL15) on the nerve electrical activity in spinal dorsal root and norepinephrine (NE) and dopamine (DA) concentrations in the paraventricular nucleus of the hypothalamus in rats with acute myocardial ischemia (AMI).

METHODS

A total of 100 male Sprague-Dawley rats were randomly divided into sham-operation, model, EA at PC6, EA at BL15 and EA at both PC6 and BL15 groups with 20 rats in each group. The nerve electrical activity in spinal dorsal roots was recorded by bipolar electrodes. NE and DA concentrations in the paraventricular nucleus of the hypothalamus were detected by high-performance liquid chromatography.

RESULTS

When compared with the sham-operation group, the nerve electrical activity in spinal dorsal roots was significantly increased while the NE and DA concentrations in the paraventricular nucleus of the hypothalamus were decreased in the model group (P<0.01). The nerve electrical activity in spinal dorsal roots was decreased and the NE and DA concentrations were increased in the paraventricular nucleus of the hypothalamus in the EA at PC6 group, the EA at BL15 group and the EA at both PC6 and BL15 group in comparison to those in the model group (P<0.01). The nerve electrical activity in spinal dorsal roots and the NE and DA concentrations in paraventricular nucleus of hypothalamus of the EA at both PC6 and BL15 group were significantly improved when compared to those of the EA at PC6 and EA at BL15 groups (P<0.05).

CONCLUSION

EA at both PC6 and BL15 acupoints exhibits the synergistic protective effects against AMI. The possible mechanism is related to regulating the nerve electrical activity in spinal dorsal roots and the concentrations of NE and DA in paraventricular nucleus of the hypothalamus.

摘要

目的

探讨电针内关（PC6）和心俞（BL15）对急性心肌缺血（AMI）大鼠脊髓背根神经电活动及下丘脑室旁核去甲肾上腺素（NE）和多巴胺（DA）浓度的影响。

方法

将100只雄性Sprague-Dawley大鼠随机分为假手术组、模型组、内关电针组、心俞电针组和内关与心俞电针组，每组20只。用双极电极记录脊髓背根神经电活动。采用高效液相色谱法检测下丘脑室旁核NE和DA浓度。

结果

与假手术组比较，模型组脊髓背根神经电活动显著增强，下丘脑室旁核NE和DA浓度降低（P<0.01）。与模型组比较，内关电针组、心俞电针组和内关与心俞电针组脊髓背根神经电活动降低，下丘脑室旁核NE和DA浓度升高（P<0.01）。与内关电针组和心俞电针组比较，内关与心俞电针组脊髓背根神经电活动及下丘脑室旁核NE和DA浓度改善更显著（P<0.05）。