[电针预处理对心肌缺血再灌注损伤大鼠下丘脑外侧区及小脑顶核中多巴胺和5-羟色胺含量的影响]
[Effect of electroacupuncture preconditioning on the contents of dopamine and 5-hydroxytryptamine in lateral hypothalamus area and cerebellar fastigial nucleus of rats with myocardial ischemia-reperfusion injury].
作者信息
Yu Qing, Cai Rong-Lin, Shao Xue-Fang, Zhang Ya-Ting, Wei Xiao-Tong, Hu Ling
机构信息
College of Acupuncture and Moxibustion and Tuina, Anhui University of CM, Hefei 230012, China; Graduate School, Anhui University of CM, Hefei 230012, China.
Research Institute of Acupuncture and Moxibustion, Anhui Academy of TCM, Hefei 230038; Anhui Key Laboratory of Acupuncture-Moxibustion Basis and Technology, Hefei 230038.
出版信息
Zhongguo Zhen Jiu. 2021 May 12;41(5):525-30. doi: 10.13703/j.0255-2930.20200509-k0001.
OBJECTIVE
To observe the effect of electroacupuncture (EA) preconditioning at heart meridian acupoints on the contents of dopamine (DA) and 5-hydroxytryptamine (5-HT) in lateral hypothalamus area (LHA) and cerebellar fastigial nucleus (FN) in the rats with acute myocardial ischemia-reperfusion injury (MIRI), and explore the role and mechanism of LHA and FN in the effect of EA at heart meridian acupoints against acute MIRI.
METHODS
Sixty SD rats were randomly divided into a sham-operation group, a model group, an EA heart meridian group and an EA lung meridian group, 12 rats in each group, as well as an LHA plus heart meridian group (damage of bilateral LHA) and an FN plus heart meridian group (damage of bilateral FN), 6 rats in each one. Three days after nucleus destruction, EA was applied to "Shenmen" (HT 7) and "Tongli" (HT 5) in the EA heart meridian group, the LHA plus heart meridian group and the FN plus heart meridian group and EA was applied to "Taiyuan" (LU 9) and "Lieque" (LU 7) in the EA lung meridian group, with 1 V in stimulating voltage and 2 Hz in frequency, lasting 20 minutes each time, once a day, for consecutively 7 days before model replication. Except in the sham-operation group, MIRI rat models were duplicated by ligation of the left anterior descending branch of the coronary artery in the rest groups. Using Power lab physiological recorder, ST segment displacement value was recorded before modeling, 30 min after ligation and 120 min after reperfusion separately. The high performance liquid chromatography-electrochemical detection and analysis system was adopted to determine the contents of DA and 5-HT in LHA and FN dialysate after rat modeling in each group.
RESULTS
In comparison of ST segment displacement value 30 min after ligation and 120 min after reperfusion among groups, the value in the model group was higher than that in the sham-operation group (<0.01), those in the EA heart meridian group, the LHA plus heart meridian group, the FN plus heart meridian group and the EA lung meridian group were lower than those in the model group successively (<0.01) and those in the EA heart meridian group were lower than those in the EA lung meridian group, the LHA plus heart meridian group and the FN plus heart meridian group successively (<0.01). The contents of DA and 5-HT in FN and LHA in the model group were lower than those in the sham-operation group (<0.01). Except in the groups with nucleus lesions, the contents of DA and 5-HT in FN and LHA of each intervention group were higher than those in the model group (<0.01), the contents of DA and 5-HT in FN in the EA heart meridian group were higher than those in the EA lung meridian group and the LHA plus heart meridian group (<0.01) and the content of 5-HT in LHA was higher than those in the EA lung meridian group and the FN plus heart meridian group (<0.01) separately, the content of DA in LHA was higher than that in the EA lung meridian group (<0.01).
CONCLUSION
EA preconditioning at heart meridian acupoints can effectively alleviate myocardial injury in acute MIRI rats, during which, DA and 5-HT in LHA and FN may be the important material basis.
目的
观察心经穴位电针预处理对急性心肌缺血再灌注损伤（MIRI）大鼠下丘脑外侧区（LHA）及小脑顶核（FN）中多巴胺（DA）和5-羟色胺（5-HT）含量的影响，探讨LHA和FN在心经穴位电针对急性MIRI保护作用中的作用及机制。
方法
将60只SD大鼠随机分为假手术组、模型组、心经电针组、肺经电针组，每组12只，另设LHA加心经组（双侧LHA损毁）和FN加心经组（双侧FN损毁），每组6只。核损毁3 d后，心经电针组、LHA加心经组和FN加心经组针刺“神门”（HT 7）、“通里”（HT 5），肺经电针组针刺“太渊”（LU 9）、“列缺”（LU 7），刺激电压1 V，频率2 Hz，每次持续20 min，每天1次，连续7 d后复制模型。除假手术组外，其余各组采用结扎冠状动脉左前降支的方法复制MIRI大鼠模型。分别于造模前、结扎后30 min、再灌注后120 min用Power lab生理记录仪记录ST段位移值。采用高效液相色谱-电化学检测分析系统检测各组大鼠造模后LHA和FN透析液中DA和5-HT含量。
结果
与假手术组比较，模型组结扎后30 min及再灌注后120 min的ST段位移值升高（P<0.01）；心经电针组、LHA加心经组、FN加心经组及肺经电针组ST段位移值均低于模型组（P<0.01），且心经电针组低于肺经电针组、LHA加心经组、FN加心经组（P<0.01）。与假手术组比较，模型组FN和LHA中DA、5-HT含量降低（P<0.01）。除核损毁组外，各干预组FN和LHA中DA、5-HT含量均高于模型组（P<0.01）；心经电针组FN中DA、5-HT含量高于肺经电针组和LHA加心经组（P<0.01），LHA中5-HT含量高于肺经电针组和FN加心经组（P<0.01），LHA中DA含量高于肺经电针组（P<0.01）。
结论
心经穴位电针预处理可有效减轻急性MIRI大鼠的心肌损伤。在此过程中，LHA和FN中的DA和5-HT可能是重要物质基础。
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