Wang Ke-Ming, Liu Jing, Wu Zi-Jian, Wang Yue-Lan, Chen Ye-Nong, He Lu, Cai Rong-Lin
Center for Researching Correlation between Meridian and Zangfu-organs, Anhui College of Chinese Medicine, Hefei 230038, China.
Zhen Ci Yan Jiu. 2011 Jun;36(3):205-8, inside back cover.
To observe the effect of electroacupuncture (EA) of "Shenmen" (HT 7), "Neiguan" (PC 6), etc. on monoamine neurotransmitter contents of hypothalamic paraventricular nucleus (PVN) region in myocardial ischemia (MI) rats, so as to analyze the substantial basis of efficacy specificity of acupoints in improving MI.
SD rats were randomized into normal control, model, EA-Shenmen (HT7), EA-Neiguan (PC 6) and EA-Taiyuan (LU 9) groups (n = 10/group). MI model was duplicated by occlusion of the anterior descending branch of the left coronary artery. EA (2 Hz, 1. 1 mA) was applied to left "Shenmen" (HT 7), "Neiguan" (PC 6) and "Taiyuan" (LU 9) for 10 min, once daily for 3 days. The hypothalamic PVN region tissue was collected for assaying the contents of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) by using enzyme linked immunosorbent assay.
In comparison with the normal control group, the contents of NE, DA and 5-HT in hypothalamic PVN in the model group were decreased significantly (P < 0.01). Compared with the model group, the contents of NE, DA and 5-HT in hypothalamic PVN in the EA-Neiguan (PC 6) and EA-Shenmen (HT 7) groups, and that of NE in the EA-Taiyuan (LU 9) group were increased significantly (P < 0.01, P < 0.05). The effects of EA-Neiguan (PC 6) and EA-Shenmen (HT 7) were significantly superior to that of EA-Taiyuan (LU 9) in increasing the contents of hypothalamic NE, DA and 5-HT (P < 0.05, P < 0.01).
Electroacupuncture of PC 6 and HT 7 can suppress MI-induced decrease of NE, DA and 5-HT contents in the hypothalamic PVN region in rats, which may contribute to its effect in resisting myocardial ischemia. The effects of EA-PC 6 and EA-HT 7 are superior to those of EA-LU 9.
观察电针“神门”（HT 7）、“内关”（PC 6）等穴位对心肌缺血（MI）大鼠下丘脑室旁核（PVN）区单胺类神经递质含量的影响，以分析穴位改善心肌缺血疗效特异性的物质基础。
将SD大鼠随机分为正常对照组、模型组、电针神门（HT7）组、电针内关（PC 6）组和电针太渊（LU 9）组（每组n = 10）。采用结扎左冠状动脉前降支的方法复制MI模型。将电针（2 Hz，1. 1 mA）施加于左侧“神门”（HT 7）、“内关”（PC 6）和“太渊”（LU 9），持续10分钟，每天1次，共3天。采集下丘脑PVN区组织，采用酶联免疫吸附测定法检测去甲肾上腺素（NE）、多巴胺（DA）和5-羟色胺（5-HT）的含量。
与正常对照组相比，模型组下丘脑PVN区NE、DA和5-HT的含量显著降低（P < 0.01）。与模型组相比，电针内关（PC 6）组和电针神门（HT 7）组下丘脑PVN区NE、DA和5-HT的含量，以及电针太渊（LU 9）组NE的含量显著升高（P < 0.01，P < 0.05）。电针内关（PC 6）和电针神门（HT 7）在增加下丘脑NE、DA和5-HT含量方面的效果显著优于电针太渊（LU 9）（P < 0.05，P < 0.01）。
电针PC 6和HT 至7可抑制MI诱导的大鼠下丘脑PVN区NE、DA和5-HT含量降低，这可能是其抗心肌缺血作用的机制之一。电针PC 6和电针HT 7的效果优于电针LU 9。
