The State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, China.
Biosens Bioelectron. 2013 Jan 15;39(1):177-82. doi: 10.1016/j.bios.2012.07.023. Epub 2012 Aug 1.
An electrochemical method for the simultaneous detection of two different tumor markers, carcinoembryonic antigen (CEA) and α-fetoprotein (AFP), in one-pot, using CdS/DNA and PbS/DNA nanochains as labels was developed. Herein, magnetic beads (MBs) as bimolecule immobilizing carriers, were used for co-immobilization of primary anti-CEA and anti-AFP antibodies. The distinguishable signal labels were synthesized by in situ growth of CdS and PbS nanoparticles on DNA chains, respectively, which were further employed to label the corresponding secondary antibodies. A sandwich-type immunoassay format was formed by the biorecognition of the antigens and corresponding antibodies. The assay was based on the peak currents of Cd(2+) and Pb(2+) dissolved from CdS and PbS nanoparticles by HNO(3) using square wave stripping voltammetry. Experimental results show that the multiplexed electrochemical immunoassay has enabled the simultaneous monitoring of CEA and AFP in a single run with wide working ranges of 0.1-100ng mL(-1) for CEA and 0.5-200ng mL(-1) for AFP. The detection limits reach to 3.3pg mL(-1) for CEA and 7.8pg mL(-1) for AFP.
采用 CdS/DNA 和 PbS/DNA 纳米链作为标记物，发展了一种在一锅反应中同时检测两种不同肿瘤标志物癌胚抗原（CEA）和甲胎蛋白（AFP）的电化学方法。在此，采用磁珠（MBs）作为双分子固定载体，用于共固定的原发性抗 CEA 和抗 AFP 抗体。通过在 DNA 链上分别原位生长 CdS 和 PbS 纳米粒子合成了可区分的信号标记物，然后将其进一步用于标记相应的二级抗体。通过抗原和相应抗体的生物识别形成三明治型免疫测定形式。该测定法基于使用 CdS 和 PbS 纳米粒子从 HNO3 中溶解的 Cd(2+)和 Pb(2+)的峰电流，采用方波溶出伏安法进行测定。实验结果表明，该多重电化学免疫测定法能够在单个运行中同时监测 CEA 和 AFP，CEA 的工作范围较宽，为 0.1-100ng mL(-1)，AFP 的工作范围为 0.5-200ng mL(-1)。CEA 的检测限达到 3.3pg mL(-1)，AFP 的检测限达到 7.8pg mL(-1)。
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