School of Biochemical and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China.
Biosens Bioelectron. 2013 Jan 15;39(1):226-30. doi: 10.1016/j.bios.2012.07.046. Epub 2012 Jul 31.
The recent development in the nanotechnology has paved the way for a large number of alloyed nanomaterials and devices of desirable properties which have useful functions for electrochemical sensor and biosensor applications. In this paper, bimetallic AuPt nanochains were synthesized through a mild chemical method, with which anti-horseradish peroxidase-conjugated anti-carcinoembryonic antigen (HRP-anti-CEA-NCAuPt) was developed for electrochemical detection of carcinoembryonic antigen (CEA) in a sandwich-type immunoassay format. The alloyed nanocrystals exhibit not only sound signal amplification effect of Au nanoparticles, but also further new combination of interfacial, electrical and structural properties arising from the disparate AuPt components. As a result, the electrochemical signal was significantly amplified by using the HRP-anti-CEA-NCAuPt as tracer and hydrogen peroxide as enzyme substrate. The linear range of the developed immunosensor is 0.01-200ng/mL and the detection limit is 0.11pg/mL of CEA, which makes the biometallic nanochains promising candidates for the next-generation sandwich-type electrochemical immunoassays.
纳米技术的最新发展为大量具有理想特性的合金纳米材料和器件铺平了道路，这些材料和器件具有电化学传感器和生物传感器应用的有用功能。本文通过温和的化学方法合成了双金属 AuPt 纳米链，并用其开发了辣根过氧化物酶偶联的抗癌胚抗原（HRP-anti-CEA-NCAuPt），用于夹心型免疫分析格式中癌胚抗原（CEA）的电化学检测。合金纳米晶体不仅具有金纳米粒子的良好信号放大效果，而且由于 AuPt 成分的不同，还具有界面、电学和结构性质的新组合。因此，使用 HRP-anti-CEA-NCAuPt 作为示踪剂和过氧化氢作为酶底物，电化学信号得到了显著放大。所开发的免疫传感器的线性范围为 0.01-200ng/mL，检测限为 0.11pg/mL 的 CEA，这使得双金属纳米链成为下一代夹心型电化学免疫分析的有前途的候选者。
