Gregory S H, Bose S K
Cancer Lett. 1979 Oct;7(6):319-24. doi: 10.1016/s0304-3835(79)80060-9.
Cell proximity has a profound effect on the glycolytic enzyme levels observed in mammalian cells grown in vitro. Hexokinase activity generally increased with the increasing cell density of both untransformed and virus-transformed cultures. In contrast, phosphofructokinase and pyruvate kinase activities were generally elevated only in crowded cultures of transformed cells. A spontaneous experimental mouse tumor cell line (EMT6) exhibited cell crowding-dependent elevations in glycolytic enzyme levels when cultured in vitro. The glycolytic enzyme levels of experimental mouse tumors grown in vivo, on the other hand, were constant and normally less than those observed in cultured EMT6 cells.
细胞 proximity 对体外培养的哺乳动物细胞中观察到的糖酵解酶水平有深远影响。己糖激酶活性通常随着未转化和病毒转化培养物的细胞密度增加而增加。相比之下，磷酸果糖激酶和丙酮酸激酶活性通常仅在转化细胞的拥挤培养物中升高。一种自发的实验性小鼠肿瘤细胞系（EMT6）在体外培养时表现出糖酵解酶水平的细胞拥挤依赖性升高。另一方面，体内生长的实验性小鼠肿瘤的糖酵解酶水平是恒定的，通常低于培养的 EMT6 细胞中观察到的水平。
