Singh V N, Singh M, August J T, Horecker B L
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4129-32. doi: 10.1073/pnas.71.10.4129.
Chick-embryo cells, transformed with Rous sarcoma virus, show enhanced rates of sugar transport and glycolysis. Determination of intracellular concentrations of glycolytic intermediates suggests that the enhanced glycolytic flux is due to increased activities of hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1), phosphofructokinase, (ATP:D-fructose-1-phosphate 6-phosphotransferase, EC 2.7.1.56), and pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40), and not directly to the increased glucose transport. This conclusion is supported by the finding that the intracellular concentration of free glucose is decreased, rather than increased, in the transformed cells. The present observations suggest that the increased glycolytic flux is related to an increased rate of phosphorylation of glucose, and that hexokinase in the transformed cells is at least partly released from its normal control mechanism involving feedback inhibition by glucose-6-P.
用劳氏肉瘤病毒转化的鸡胚细胞显示出糖转运和糖酵解速率增强。对糖酵解中间产物细胞内浓度的测定表明，糖酵解通量的增强是由于己糖激酶（ATP：D-己糖6-磷酸转移酶，EC 2.7.1.1）、磷酸果糖激酶（ATP：D-果糖-1-磷酸6-磷酸转移酶，EC 2.7.1.56）和丙酮酸激酶（ATP：丙酮酸2-O-磷酸转移酶，EC 2.7.1.40）活性增加，而不是直接由于葡萄糖转运增加。这一结论得到以下发现的支持：在转化细胞中，游离葡萄糖的细胞内浓度降低而非升高。目前的观察结果表明，糖酵解通量的增加与葡萄糖磷酸化速率的增加有关，并且转化细胞中的己糖激酶至少部分地从其涉及由6-磷酸葡萄糖反馈抑制的正常控制机制中释放出来。
