Jayawardane G W, De Alwis M C, Bandara D
Veterinary Research Institute, Gannoruwa, Peradeniya, Sri Lanka.
Aust Vet J. 1990 Oct;67(10):364-6. doi: 10.1111/j.1751-0813.1990.tb07405.x.
An international effort (sponsored by the Australian Centre for International Agricultural Research) is being made to develop oral vaccines that will protect village chickens against Newcastle disease. The vaccines being used are derivatives of the avirulent Australian V4 strain that have been selected for enhanced heat resistance. The present study, undertaken in Sri Lanka, used local processed (parboiled) rice as a vehicle for the vaccine. Chickens receiving two doses of vaccine on cooked, parboiled rice were completely protected against contact challenge with the virulent SL 88/1 Sri Lankan strain of Newcastle disease virus Chickens kept in contact with these vaccinated chickens were similarly protected. Lower levels of protection were achieved with vaccine given on uncooked parboiled rice. V4 vaccine administered intranasally also gave complete protection. Serums from vaccinated chickens that survived challenge were tested for haemagglutination-inhibition antibodies, using both vaccine virus and challenge virus as antigens. Titres were higher against vaccine virus.
一项国际合作努力（由澳大利亚国际农业研究中心资助）正在进行，旨在研发能保护乡村鸡群免受新城疫侵害的口服疫苗。所使用的疫苗是无毒澳大利亚V4株的衍生物，这些衍生物已被选用于增强耐热性。在斯里兰卡进行的本研究使用当地加工（煮过的）大米作为疫苗载体。在煮熟的煮过的大米上接受两剂疫苗的鸡完全受到保护，免受强毒力的斯里兰卡新城疫病毒SL 88/1株的接触攻击。与这些接种疫苗的鸡接触的鸡也同样受到保护。用未煮熟的煮过的大米接种疫苗所获得的保护水平较低。经鼻内接种V4疫苗也能提供完全保护。对接种疫苗后经攻击存活的鸡的血清进行了血凝抑制抗体检测，使用疫苗病毒和攻击病毒作为抗原。针对疫苗病毒的滴度更高。
