通过理性的基因组挖掘发现来源于大豆根瘤菌 USDA6 的新型（+）-γ-内酰胺酶。
Discovery of a novel (+)-γ-lactamase from Bradyrhizobium japonicum USDA 6 by rational genome mining.
机构信息
State Key Laboratory of Chemical Resources Engineering, Beijing University of Chemical Technology, Beijing, People's Republic of China.
出版信息
Appl Environ Microbiol. 2012 Oct;78(20):7492-5. doi: 10.1128/AEM.01398-12. Epub 2012 Aug 10.
Abstract
A novel (+)-γ-lactamase used for the resolution of racemic γ-lactam from Bradyrhizobium japonicum USDA 6 was found as a result of sequence-structure guided genome mining. It consists of 409 amino acids, only 49% of which are identical to the amino acid sequences of the known (+)-γ-lactamase from Sulfolobus solfataricus. This is only the third (+)-γ-lactamase gene to be reported.
摘要
一种新型（+）-γ-内酰胺酶，用于拆分来自大豆根瘤菌 USDA 6 的外消旋 γ-内酰胺，是通过序列-结构导向的基因组挖掘发现的。它由 409 个氨基酸组成，只有 49%与已知的来自 Solfolobus solfataricus 的（+）-γ-内酰胺酶的氨基酸序列相同。这是仅有的第三个被报道的（+）-γ-内酰胺酶基因。
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