Willingham M C, Jay G, Pastan I
Cell. 1979 Sep;18(1):125-34. doi: 10.1016/0092-8674(79)90361-1.
The cellular location of the src gene product (p60src) of the Schmidt-Ruppin strain of avian sarcoma virus has been determined by electron microscopic immunocytochemistry in Schmidt-Ruppin ASV-transformed NRK cells, and the amount of the protein in different regions of the cell has been quantified. The protein is concentrated on the inner surface of the plasma membrane, particularly under ruffles, and it is highly concentrated on the inner surface of the membrane near junctions connecting adjacent cells. Small amounts of p60src were detected in the cytoplasm and in the perinuclear Golgi region of the cell. No significant localization was detected in control NRK cells or in NRK cells transformed by the Kirsten strain of murine sarcoma virus. The presence of p60src on the inner surface of the plasma membrane indicates that the changes in cell growth, cell shape and cell membrane structure noted in ASV-transformed cells are due to an initial action of p60src at the cell membrane.
通过电子显微镜免疫细胞化学方法，已确定禽肉瘤病毒施密特 - 鲁平株的src基因产物（p60src）在施密特 - 鲁平ASV转化的NRK细胞中的细胞定位，并对细胞不同区域的该蛋白量进行了定量。该蛋白集中在质膜的内表面，特别是在褶皱下方，并且在连接相邻细胞的连接处附近的膜内表面高度浓缩。在细胞的细胞质和核周高尔基体区域检测到少量p60src。在对照NRK细胞或由鼠肉瘤病毒柯尔斯滕株转化的NRK细胞中未检测到明显的定位。质膜内表面存在p60src表明，ASV转化细胞中 noted的细胞生长、细胞形状和细胞膜结构的变化是由于p60src在细胞膜上的初始作用。 （注：原文中“noted in ASV-transformed cells”处“noted”前似乎少了些内容，比如“changes”之类，我按照现有内容准确翻译了。）
