Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai, 200031, China.
Protein Cell. 2012 Sep;3(9):691-700. doi: 10.1007/s13238-012-2039-0. Epub 2012 Aug 12.
Valid animal models are useful for studying the pathophysiology of specific disorders, such as neural disease, diabetes and cancer. Previous molecular phylogeny studies indicate that the tree shrew is in the same order as (or a close sister to) primates, and thus may be an ideal model in which to study human disease. In this study, the proteome of liver and muscle tissue in tree the shrew was identified by combining peptide fractionation and LC-MS/MS identification. In total, 2146 proteins were detected, including 1759 proteins in liver samples and 885 proteins in skeletal muscle samples from the tree shrew. Further sub-source analysis revealed that nearly half of the identified proteins (846 proteins and 418 proteins) were derived from human database. In this study, we are the first to describe the characteristics of the proteome from the liver and skeletal muscle of the tree shrew. Phylogenetic tree analysis based on these proteomic data showed that the tree shrew is closer to primates (human) than to glires (the mouse and rat).
有效的动物模型对于研究特定疾病的病理生理学非常有用，如神经疾病、糖尿病和癌症。先前的分子系统发育研究表明，树鼩与灵长类动物（或灵长类动物的近亲）在同一目，因此可能是研究人类疾病的理想模型。在这项研究中，通过肽分级和 LC-MS/MS 鉴定相结合，鉴定了树鼩肝脏和肌肉组织的蛋白质组。总共检测到 2146 种蛋白质，其中包括 1759 种在肝脏样本中，885 种在骨骼肌样本中。进一步的亚源分析表明，近一半的鉴定蛋白（846 种蛋白质和 418 种蛋白质）来源于人类数据库。在这项研究中，我们首次描述了树鼩肝脏和骨骼肌蛋白质组的特征。基于这些蛋白质组数据的系统发育树分析表明，树鼩与灵长类动物（人类）的亲缘关系比与啮齿类动物（老鼠和大鼠）更近。
