Weiner D, Wolf B
Department of Human Genetics, Medical College of Virginia, Richmond 23298.
Biochem Med Metab Biol. 1990 Dec;44(3):271-81. doi: 10.1016/0885-4505(90)90072-9.
Biotin uptake was studied in isolated cultured hepatocytes of normal and biotin-deficient rats. Biotin uptake was temperature-dependent with respect to physical, but not to chemical, processes, proportional to the exogenous biotin concentration in the medium, independent of pH and sodium ion concentrations of the medium, and uneffected by the presence of structural analogues of biotin or metabolic inhibitors in both normal and biotin-deficient hepatocytes. These results suggest that biotin uptake occurs by a passive, nonmediated, non-energy-dependent mechanism in rat hepatocytes.
在正常和生物素缺乏大鼠的离体培养肝细胞中研究了生物素摄取。生物素摄取在物理过程方面依赖于温度，但在化学过程方面并非如此，与培养基中外源生物素浓度成正比，与培养基的pH值和钠离子浓度无关，并且在正常和生物素缺乏的肝细胞中均不受生物素结构类似物或代谢抑制剂存在的影响。这些结果表明，大鼠肝细胞中生物素摄取是通过被动、非介导、非能量依赖的机制发生的。
